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SepSphere™ Immobilization Kits

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Biopolymers and small molecules immobilized on solid support are important reagent-tools for biotech research. An antigen immobilized on a solid support, such as agarose bead, magnetic bead, or polymer bead is often used as affinity purification media. Immobilized antigen can also be used as a capture tool for protein/marker identification in drug discovery or as a diagnostic reagent in immunoassay. The development of techniques and methods for immobilization is essential for many biotechnological advancements.

Two types of solid supports are developed at CellMosaic

  • Conventional hydrophilic agarose beads functionalized with cyanate esters or imidocarbonates (CNBr activated), amino, carboxylic acid, glyoxy, glutaldehyde, aminooxy, pyridyl disulfide, maleimide, or epoxide for the convenience of immobilization using the customer’s own compound
  • Novel hydrophilic AqT™ coated beads.

Three Essential Components of a Successful Immobilization Kit

  • Quality and stability of the functionalized beads - CellMosaic has tailored the loading capacity and stability of the agarose beads for each application.
  • Chemistry design - CellMosaic’s kit comes with pre-made buffers with simple easy to follow direction.
  • Immobilization workflow - We have developed a universal workflow and kit design that allows immobilization, washing of unreacted starting materials, and column packing all in one column with a simple set-up in any lab. The immobilization efficiency in the column is the same as if performed in a regular reaction vessel. The washing is performed with a simple manual air push mechanism using a syringe and requires no gas/air flow or vacuum. For more information, please click here.

CellMosaic currently provides the following SepSphere™ immobilization kit using agarose beads, and we are adding additional kits configurations as needed. Please inquire if you do not see your current configuration of interest listed or request a custom immobilization service if you are interested in using a solid support other than agarose beads.

SepSphere™ Immobilization Kits  Selection Guide

Ligand Reactive Groups
Agarose Reactive Groups
Stability of Linkage
CM71542 Antibody/ Protein/Enzyme -NH2 -C(=O)H (glyoxyl) Secondary amine  Stable 
CM71543 Antibody/ Protein/Enzyme -NH2  Glutaraldehyde  Imine  Stable at pH 5-9
CM71301 Peptide -SH (Cys thiol) Maleimide Thiol ether  Stable 
CM71302 Peptide -SH (Cys thiol) -S-S-Pyridine Disulfide bond Stable (can be released under reducing conditions)
CM71303 Peptide -NH2 -COOH Amide bond  Stable 
CM71401 Oligo, DNA -NH2 -COOH Amide bond Stable 
CM71402 Oligo, DNA -NH2 -CH(O)CH2 (epoxide) β-amino alcohol Stable 
CM71403 Oligo, DNA -OPO3 (phsophate) -COOH Amide bond  Stable 
CM71004 Small molecule -COOH -NH2 Amide bond  Stable 
CM71005 Small molecule -NH2 -C(=O)H (glyoxyl) Secondary amine  Stable 
CM71006 Small molecule -NH2 -CH(O)CH2 (Epoxide) β-amino alcohol Stable 
CM71007 Small molecule -OH (Aliphatic alcohol) -NH2 Thiol ether Stable 
CM71008 Small molecule -NH2 -COOH Amide bond  Stable 
CM71010 Small molecule -C=O- (Ketone or aldehyde) -ONH2 (aminooxy) Oxime bond  Stable (can be released at acidic or basic conditions)