CellMosaic® has designed this personalized immobilization kit for small molecule containing amine functional group or phenol group (aromatic OH group). This kit is custom made to order. Some of the small molecules may contain other functional groups that can interfere with the reaction. We recommend the customer to email your information of your small molecule before placing this order.  

The user supplies the small molecule amine or phenol. Using the kit components, the customer immobilizes the small molecule onto SepSphere™ glyoxyl agarose in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

• Minimum requirement for immobilization set-up
• All reagents, buffers, and plasticwares are included 
• Stable attachment
• Easy preparation with air push mechanism for washing: less than 2 h hands-on time

 Specification of the Solid Support

• Functional groups: Epoxide
• Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
• Particle size: 45-165 um 
• Mean bead size: 90 um   
• Ligand concentration: ~2 μmol epoxide per mL agarose

 Specification of the Immobilized Product

• Immobilized chemistry: immobilized via ether (for phenol) or secondary amine (for amine)
• Chemical stability: stable 
• pH Stability: stable     

 Requirement for Small Molecule

• Preferably >90% pure by C18 HPLC
• Total amount: 1-10 mg 
• Stable at pH 10.0