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Antibody-Drug Conjugate (ADC) Kits

 

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ADC FLYER
APPLICATION NOTE

CellMosaic ADC PerKits™ are available to prepare antibody conjugates through surface amines or reduced thiols. Some ADC kits apply to general payloads with certain functional groups. Some ADC kits supply the selected, standard linkage molecules conjugated to a selected payload, ready with the appropriate reactive modifications to directly attach the payload and linker to your specific antibody of interest. The kits are complete with all reagents and procedures for attaching the selected payload directly to your antibody and purifying the final ADC. All you need to supply is your antibody and standard lab equipment.

A typical CellMosaic personalized ADC kit results in the preparation of approximately 2-3 mg of final ADC in buffer with an average DAR of 2-4. Purities are typically >90% for the conjugates free of or with less than 5% of unreacted payload. Individual results can vary depending on the specific antibody system you are using and the hydrophobicity of your payload. Kit instructions are complete and easy to follow, and can typically be carried out within 6 hours (with less than 1-hour hands-on time).

AqT™ ADC Evaluation Kits: In the event that an drug conjugate cannot be made using classical linkers or the effectiveness of the drug conjugates are compromised by the number of toxins loaded onto the antibody using CellMosaic PerKits™ ADC, CellMosaic has developed super-hydrophilic and water soluble AqT™ linker for this purpose. Limited quantities of  AqT™ADC Evaluation Kits are currently available for customer to perform conjugations in-house and evaluate the benefit of AqT™ linkers for ADC on a small scale. Click here for more information.

Other products on the market for antibody screening may utilize a general secondary antibody, or a general antibody affinity coupling (such as protein-A), to provide indirect conjugation, but these indirect conjugation approaches for ADCs can significantly impact the hydrodynamic size and physiological behaviors (for example, changing cleavage and degradation mechanisms), so these products do not provide a fully representative approach for evaluating your novel ADC in screening or for proof-of-concept (POC) studies.


Perkit™ ADC Selection Guide

 Product#

 Protein

 Drug

 Scale of  Reaction

 Labeling Chemistry

 Linkage

 CM11429 IgG Ab No drug (VC-PAB linker only) 1-3 mg Reduced thiol Releasable
 CM51403  IgG Ab  Acid  1-3 mg  Surface amine  Stable 
 CM11410 (x3)  IgG Ab  DM1  1-3 mg  Surface amine  Stable 
 CM11406 (x3)  IgG Ab  Doxorubicin  1-3 mg  Surface amine  Stable 
 CM11407 (x3)  IgG Ab  Methotrexate  1-3 mg  Surface amine  Stable 
 CM11409 (x3)  IgG Ab  MMAE  1-3 mg  Reduced thiol  Releasable 
 CM11422 (x3)  IgG Ab  MMAF  1-3 mg  Reduced thiol  Stable 
 CM11425 (x3)  IgG Ab  MMAF  1-3 mg  Reduced thiol  Releasable 
 CM11408 (x3)  IgG Ab  SN38  1-3 mg  Surface amine  Releasable 
 CM11416 (x3)  F(ab')2  MMAE  0.73-2.2 mg  Reduced thiol  Releasable 

 CM11419 (x3)

 F(ab')2  DM1  0.73-2.2 mg  Surface amine  Stable

(Note: additional kits configurations can be added depending on the request from customers. Please inquire if you do not see your current configuration of interest listed or request a custom bioconjugation service)