This is a personalized conjugation kit (PerKit™) for antibody (IgG) labeling of a small molecule containing carboxylic acid (-COOH) functional groups. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

This kit provides materials to label 1 to 3 mg of one antibody sample (IgG).

If you have less than 1 mg of antibody, please add Antibody PerKit™ Customization for Lower Amount (AS1003) along with this kit. 
If you would like CellMosaic to perform the conjugation instead, please add PerKit™ Custom Conjugation (AS1002) along with this kit.

Shipping policy: Ship at RT, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Requirement for Small Molecule

• Preferably > 90% pure by C18 HPLC
• Total amount: 20 µmol 
• Absence of primary or secondary amine (-NH2) groups
• Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
• For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic for custom conjugation service.

Requirement for Antibody (IgG)

• Preferably > 90% pure by gel electrophoresis
• Total amount: 1-3 mg protein content as measured by UV

Key Features of this PerKit™

• Offers a simple and easy way to label IgG with any small molecule containing carboxylic acid
• Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
• Offers in-process optimization of the degree of loading during the conjugation
• All reagents and supplies included for preparation and purification
• Over 90% pure conjugated products by SEC 
• Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and calculation of the loading.

Labeling Chemistry

Schematic diagram of the workflow for preparing antibody-small molecule conjugates starting with 3 mg of IgG (volume of reagents varies if the amount of IgG is < 3 mg)