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PerKit™ Antibody Small Molecule Acid Conjugation Kit

  • CM51403 Antibody small molecule acid conjugation kit
Price:
$669.00
SKU:
CM51403
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
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SDS USER MANUAL

This is a personalized conjugation kit (PerKit™) for antibody (IgG) labeling of a small molecule containing carboxylic acid (-COOH) functional groups. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

This kit provides materials to label 1 to 3 mg of one antibody sample (IgG).

If you have less than 1 mg of antibody, please add Antibody PerKit Customization for Lower Amount (AS1003) along with this kit. 
If you would like CellMosaic to perform the conjugation instead, please add PerKit Custom Conjugation (AS1002) along with this kit.

Shipping policy: Ship at RT, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Requirement for Small Molecule

  • Preferably > 90% pure by C18 HPLC
  • Total amount: 20 µmol 
  • Absence of primary or secondary amine (-NH2) groups
  • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
  • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic for custom conjugation service.

Requirement for Antibody (IgG)

  • Preferably > 90% pure by gel electrophoresis
  • Total amount: 1-3 mg protein content as measured by UV

Key Features of this PerKit™

  • Offers a simple and easy way to label IgG with any small molecule containing carboxylic acid
  • Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
  • Offers in-process optimization of the degree of loading during the conjugation
  • All reagents and supplies included for preparation and purification
  • Over 90% pure conjugated products by SEC 
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and calculation of the loading.


Labeling Chemistry

CM51403 antibody small molecule acid conjugation kit 


Schematic diagram of the workflow for preparing antibody-small molecule conjugates starting with 3 mg of IgG (volume of reagents varies if the amount of IgG is < 3 mg)

cm51403-antibody-small-acid-labeling-protocol.png

Other Details

Reaction Scale:
1-3 mg
Antibody Labeling Sites:
Surface amines
Antibody Drug Linkage:
Stable amide bond

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