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SDS | USER MANUAL |
This is a personalized conjugation kit (PerKit™) for antibody (IgG) labeling of a small molecule containing carboxylic acid (-COOH) functional groups. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid.
This kit provides materials to label 1 to 3 mg of one antibody sample (IgG).
If you have less than 1 mg of antibody, please add Antibody PerKit™ Customization for Lower Amount (AS1003) along with this kit.
If you would like CellMosaic to perform the conjugation instead, please add PerKit™ Custom Conjugation (AS1002) along with this kit.
Shipping policy: Ship at RT, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.
Requirement for Small Molecule
Requirement for Antibody (IgG)
Key Features of this PerKit™
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and calculation of the loading.
Labeling Chemistry
Schematic diagram of the workflow for preparing antibody-small molecule conjugates starting with 3 mg of IgG (volume of reagents varies if the amount of IgG is < 3 mg)