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SDS | USER MANUAL |
This is a personalized conjugation kit (PerKit™) for antibody (IgG) labeling of a small molecule containing carboxylic acid (-COOH) functional groups (3 mg scale). Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid.
This kit provides materials to label one antibody. Scale of the reaction: 3 mg.
Requirement for Small Molecule
Key Features of this PerKit™
Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendations for the conjugation if your molecule has special features.
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.
Protocol
Labeling Chemistry