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    • NeIon™ Technologies

    NeIon™ technologies use specifically designed organotrifluoroborate compounds to label analytes of interest, enhancing their detection sensitivity in negative-ion-mode mass spectrometry (MS). NeIon™ technologies can also support the quantification of trace levels of these analytes.

    Organotrifluoroborates are stable, permanently negatively charged compounds that can serve as effective MS tags because of the following properties:

    • Permanent negative charge: Their charge is independent of pH, unlike phosphate, sulfonate, and carboxylate groups, which can improve ionization efficiency and detection sensitivity in negative ion mode.
    • Predictable fragmentation: NeIon™ tags can produce or induce predictable fragmentation patterns in MS/MS analysis, improving analytical selectivity.

    Example of a NeIon™ Tag

    Example chemical structure of a NeIon mass spectrometry tag

    Why Use Negative Ion Mode?

    Negative Ion Mode Analysis

    • Relatively few MS analyses are performed in negative ion mode because only a limited number of molecules readily form stable negative ions.
    • Selective labeling of analytes with a negative-ion MS tag can substantially improve sensitivity, potentially by 100- to 1,000-fold, through enhancement of the signal-to-noise ratio.
    • To the best of CellMosaic’s knowledge, no other MS tag has been specifically designed to improve both sensitivity and selectivity in negative ion mode.

    Positive Ion Mode Analysis

    • Most MS analyses are performed in positive ion mode because many small and large molecules ionize readily under positive-ion conditions.
    • The broad ionization of many compounds can increase background signals, matrix interference, and spectral complexity.
    • Sensitivity-enhancing mass tags have been developed for positive ion mode, but the associated intellectual property landscape is highly crowded.

    Comparison of positive and negative ion modes in mass spectrometry

    Fragmentation Pattern of NeIon™ Tags

    NeIon™ tags are designed to produce characteristic fragmentation patterns during tandem mass spectrometry. These predictable product ions can improve analyte identification and analytical selectivity.

    Representative fragmentation pattern of a NeIon mass spectrometry tag

    Multiplexing with NeIon™ Tags

    Structurally distinct NeIon™ tags can be designed to generate distinguishable mass or fragmentation signatures, enabling multiplexed analysis of multiple analytes within a single MS experiment.

    Multiplexed mass spectrometry analysis using NeIon tags

    Patent and Licensing Information

    Patent Protection

    NeIon™ technologies are covered by U.S. Patent Nos. 9,346,833 and 9,518,067, owned by CellMosaic, Inc.

    Research-Use License

    The purchase of NeIon™ products includes a limited license for internal research and development use only. The purchase does not grant rights for commercial use.

    Commercial-Use Restrictions

    Commercial use includes, but is not limited to:

    1. The sale, lease, license, or other transfer of the material or any material derived from or produced using it.
    2. The sale, lease, license, or other grant of rights to use the material or any material derived from or produced using it.
    3. The use of the material to perform services for a fee on behalf of third parties, including contract research, drug screening, or related commercial services.

    Commercial Licensing

    Organizations interested in using NeIon™ reagents for commercial purposes should contact CellMosaic for licensing information.