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HPLC Analysis
At CellMosaic, we have extensive experience in the analysis and purification of small organic molecules, biopolymers, and bioconjugates using high-performance liquid chromatography (HPLC) across a range of media and column chemistries. We have established robust methods for analyzing peptides, oligonucleotides, proteins, antibodies, and their conjugates. For projects involving complex or specialized molecules, we encourage consultation with our specialists to develop tailored HPLC methods.
Reverse-Phase HPLC (RP-HPLC)
Reverse-phase HPLC is widely used for the analysis of small organic molecules, peptides, oligonucleotides, proteins, antibodies, enzymes, and their conjugates. We support method development for bioconjugates, including proteins, peptides, oligos, and antibody–drug conjugates (ADCs). Our platform includes a range of column chemistries (C4, C8, C18, and phenyl), enabling efficient optimization of separation conditions based on analyte properties.
Size Exclusion Chromatography (SEC)
SEC is suitable for analyzing mixtures of biopolymers by separating components based on their size or apparent molecular weight. Larger molecules elute earlier than smaller ones. This method is commonly used to estimate molecular weight and, in bioconjugate analysis, to assess the presence of unconjugated species and the degree of aggregation within a sample.
Hydrophobic Interaction Chromatography (HIC)
HIC separates proteins and antibody–protein conjugates based on hydrophobicity. While conceptually similar to reverse-phase chromatography, HIC uses a less hydrophobic stationary phase, allowing separations under more aqueous, less denaturing conditions. It is particularly useful for evaluating drug loading and heterogeneity in antibody–drug conjugates (ADCs) and other protein–drug conjugates.
Ion Exchange Chromatography (IEX)
Anion and cation exchange chromatography separate molecules based on their net charge. This approach provides a mild and selective method for analyzing peptides, oligonucleotides, proteins, and their conjugates. It is especially valuable for resolving charge variants and characterizing molecular heterogeneity.