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HIC HPLC Analysis of Biopolymer, Conjugate, and ADC

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* Please fill out your sample info: Name, Lot#, Description, and Conc.:

Hydrophobic Interaction Chromatography (HIC) HPLC is good for analyzing protein or antibody/protein bioconjugate based on their hydrophobicity. The theory behind hydrophobic interaction chromatography is similar to reverse-phase chromatography; however, the degree of substitution of HIC adsorbents is diluted compared to reverse-phase chromatography. Thus, the compound can be eluted in a more polar and less denaturing environment. For example, HIC has been used extensively to analyze drug loading in antibody-drug or protein-drug conjugatesA standard pdf file of the HPLC profile will be supplied.

Per sample charge.

Setup fee of $250 applies per batch analysis. Setup fee will be waived if the conjugate is made using CellMosaic's kit.

Standard services is 1-3 days.

Sample submission for biopolymer, bioconjugate, and ADC:

1)      Dilute your samples in PBS buffer to 1 mg/mL, and then transfer minimum 50 μL of the diluted solution to a 500 μL micro-centrifuge tube. Label the vial properly.

2)      Ship your samples with a cold pack for overnight delivery.

For analysis of bioconjugates such as antibody drug conjugates, always ship a sample of un-labeled biopolymer for analysis at the same time.

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