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ADC and ODC Related Questions

CellMosaic provides antibody drug conjugate (ADC) and other drug conjugate (ODC) related products and services. The following lists some of the frequently asked questions regarding the general applications and usages of these products. For specific product questions, please click here. For service related questions, please click here.  

How many antibodies that I can label with CellMosaic’s ADC kit?

CellMosaic’s ADC kit is configurated by the number of labeling reactions, not the number of antibodies. In the “Number of Reactions” on the website under each kit product, if you choose 1, you will get the price and catalog number for 1-reaction kit (x1). If you choose 3, you will get the price and catalog number for a 3-reaction kit (3x). The 3-reactions kit is usually around 20% cheaper compared to the 1-reaction kit.

What is the difference between ordering one 3-reaction kit or three 1-reaction kit?

The 1-reaction kit is good if you want to conduct 3 labeling reactions at different time points. The 3-reaction kit is around 20% cheaper than the 1-reaction kit and it is good if you want to conduct 3 labeling reactions simultaneously or within a short time frame as the buffers are packaged in the same tube for all 3 reactions.

I have only a small amount of antibody, can I split the reagents for multiple labeling?

Unfortunately, No. The ADC kit only comes with either one or three sets of purification and buffer sets.

What are the options for PerKit™ customization?

CellMosaic’s kit is usually optimized and configurated for labeling/conjugating around certain types of molecules with certain requirements for molecule weight, amount, and loading. If your labeling specifications are outside these parameters (e.g. other type of antibodies, different MW, lower amount, or require higher loading), we offer customization (PerKit™ customization) of the standard kit that you can choose directly on the web order. The cost for each customization is $200 as of June 2024. Customization may require protocol modifications or replacement of some kit components.

If you cannot find the customization you need, please email info@cellmosaic.com with the product number and your customization need. Some customizations may not be feasible with the standard kit. In that case, we offer custom bioconjugate services instead.

Does CellMosaic sell protein-drug conjugation kits that work with Single-Domain Antibodies (~15KDa)?

Yes, we currently have 4 PerKit™ Products that can be modified to accommodate a 10-20 KDa antibody. Protein Mertansine (DM1) Conjugation Kit (CM11414), Protein MMAE Conjugation Kit (CM11413), Protein Deruxtecan Conjugation Kit (CM11432), Protein SN38 Conjugation Kit (CM11430). If the kit you would like to modify is not listed above, please contact us to see if we can find you a custom solution.

How do I properly dispose of the hazardous waste from my ADC or PDC conjugation kit?

The best way to dispose of hazardous waste from using our kits is to put used columns and emptied tubes of reagents in the plastic waste bag provided and close it. Dispose the entire waste bag as solid lab waste according to local regulations. We do not recommend treating hazardous waste without consulting your EHS specialists.

I am worried about the solubility of our antibodies; Can I substitute PBS for the buffers included in my ADC conjugation kit?

Unfortunately, you cannot substitute PBS for the buffers in our kits. The buffer systems we have designed for the kits are key to the success of your conjugation. The buffer systems have been extensively tested and used by our customers. We have not seen any precipitation or solubility issues caused by these buffers. However, if you have an antibody that its properties are quite different from a conventional one and are concerned with the solubility, you can contact us with any suggestions.

What can I do to reduce aggregation of my ADC?

Aggregation is generally caused by the loading or stacking of hydrophobic drugs on your ADC. CellMosaic’s ADC kits use clinical approved conventional linkers such as VC-PAB, ethylene type for proof-of-concept studies. These conventional linkers add the hydrophobicity to ADC and aggregate the problem. To reduce the aggregation in ADC, you can use a more water-soluble drug or load the drug with a less hydrophobic linker such as our super hydrophilic AqueaTether®(AqT®) linker. If you do not want to change the configuration of your ADC, you can try to lower the number of drugs loaded per antibody. For long term storage of your ADC, we recommend using our ADC Stabilizing Buffer which helps to keep the aggregation low during storage.

Which kit should I use to conjugate a ~50Kda Protein?

You can use any of the protein drug conjugation kits. We currently offer protein drug conjugation kits for DM1, SN38, MMAE, Deruxtecan, and other small molecules containing one carboxylic acid group. Additional kits configuration can be added depending on the request from customers. Please inquire if you do not see the drugs listed or request a custom conjugation service.

Note: With a smaller protein/antibody the amount of the drugs you can load without too much aggregation is limited. For further information on this topic see: ‘What can I do to reduce aggregation of my ADC?’

Can CellMosaic’s antibody drug conjugation kits be used to conjugate nanobodies instead of antibodies?

No, CellMosaic’s kits that include “antibody” in the name are specifically designed for use with IgG1 type antibodies with MW of ~150 KDa. However, CellMosaic has protein-drug conjugation kits that may be used for nanobody depending on the MW of your nanobody. If your nanobody has a MW of ≤20 KDa, you may need some customization of these kits or request custom conjugation services. We can also provide a custom conjugation service in which we make an initial batch of your nanobody drug conjugate and use that synthesis process to design a kit for you for future use.

What concentration should I use for ADC Controls (i.e., CM51100, CM51101, CM51102, CM11429) in my model?

As a general rule for any control usage, you should use the same drug concentration as your binding ADC (taking into consideration the DAR). The concentration of binding ADC you use will depend on your application. For Cell Viability Studies to determine the IC50, you can use various concentrations ranging from 0.001 ug/mL to 10 ug/mL. These non-binding ADC controls can also cause some cell death in a non-specific way. Keep this in mind when choosing your control.

In addition, if you need a negative control for linker only, you can conjugate your antibody with a VC-PAB linker without toxin, a different control option can be made using our control kit CM11429 which prepares a binding antibody without the toxin.