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Protein MMAE Conjugation Kit

  • Protein MMAE conjugate CM11413
  • Mal-VC-PAB-MMAE structure
Custom kit made to order, usually ships within 1 week
Calculated at checkout


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CellMosaic® has designed this personalized conjugation kit to work with any protein with a MW over 20 KDa. The customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a thiol-protein, followed by reaction of the maleimide-activated VC-PAB-MMAE to generate the protein-MMAE conjugates (a similar method developed by Seattle Genetics: Sun et al.2005,Bioconjugate Chem. 16, 1282-1290). One step purification typically provides the resulting protein-MMAE at greater than 95% purity. 

This kit provides materials to label either one or three proteins. Reaction scale: 20 nmol 

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Requirement for protein:

  • Amount: 20 nmol (1 mg if the MW of protein is 50KDa) (Lower amount of protein is fine, but need customization. Please contact us for the customization)
  • MW: >20 KDa (Lower MW is fine, but may need customization or custom synthesis. Please contact us for any suggestion)
  • Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
  • Protein has some lysine residues

Drug Information

  • NameMonomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
  • CAS number646502-53-6
  • Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
  • Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

Key Features of this PerKit™

  • Offers a simple and easy way to label protein with MMAE with minimum exposure to the toxin
  • Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
  • Fast and easy preparation: 6 h preparation and <2 h hands-on time
  • All reagents and supplies included for preparation and purification
  • Average 2-4 MMAE labeling per protein 
  • Options to choose tailored services at CellMosaic after conjugation

Prior to conjugation: you can supply your protein information when you place your order. CellMosaic will give recommendations for the conjugation if your protein has special features.

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DOL.


Related Products

Thiol Assay Kit (Cat. CM90004)The average free thiol groups per protein is 4 after thiol modification. If necessary, you can purchase this kit separately from CellMosaic to measure the free thiols. Total assay time is 20 minutes.

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