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Hydrophobic Interaction Chromatography (HIC) Buffer Set

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Product Description

HIC HPLC analysis is a useful tool to separate bioconjugates labeled with very hydrophobic small molecules based on their hydrophobicity. The hydrophobic interaction of the bioconjugates and the column media is affected by the presence of salts or organic solvent in the running buffer.

CellMosaic has designed this high quality HIC buffer set for your convenient analysis of bioconjugates, such as antibody-drug conjugate (ADC) and protein or peptide-drug conjugate following the preparation of the bioconjugates using CellMosaic’s Personalized Conjugation Kit™ (PerKit™).

This HIC buffer set comes with two buffers and instruction for usage. Buffer A has high salt and Buffer B contains no salt. A high salt concentration aids the interaction of the bioconjugates with the column media. Usually, a decreasing salt gradient is used to elute samples from the column. As such, the lowest degree of labeled bioconjugate (low hydrophobicity) is eluted first and the most labeled bioconjugates elute last.

Quality and Convenience

  • Experimentally selected ultra-pure components for minimum UV background absorbance.
  • Sterile filtrated & bottled to prevent any analyte degradation during the run
  • Can be used with any standard HIC column for biopolymer analysis
  • Each buffer set is sufficient for 30 analysis.

Application of the product:

  • Analyze antibody-drug conjugates
  • Analyze protein and peptide-drug conjugates
  • Analyze other biopolymers labeled with very hydrophobic small molecules

Reference: Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products. Szabolcs Feketea; Jean-Luc Veuthey; Alain Beck; Davy Guillarme. Journal of Pharmaceutical and Biomedical Analysis 130 (2016) 3–18. 

Key Components

Two buffer bottles per package. 


Part #



Buffer A (Red label)


250 mL

0.1M Sodium phosphate buffer, 1.8 M Ammonium sulfate, pH 7.0

Buffer B (Blue label)


200 mL

0.1M Sodium phosphate buffer, pH 7.0


Other Details

Number of analysis:

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