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    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
    • Our Customers
    • Our Team
    • News
  • Contact Us
  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
    • ADC Kits List
    • Routine ADC Synthesis
    • ADC Controls
    • ADC Analysis
    • Buffers and others
  • Drug Conjugation Kits and Solutions
    • Protein-Drug Conjugate (PDC) Kits
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    • Oligo Labeling and Conjugation Kits
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    • Others
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    • ADC Controls
    • ADC Analysis
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    • Protein-Drug Conjugate (PDC) Kits
  • Personalized Conjugation Kits (PerKit™)
    • Immobilization Kits
    • Oligo Labeling and Conjugation Kits
    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
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    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
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    • Analytical Standards
    • Assay Kits
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    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
  • AqueaTether™ (AqT™) Reagents and kits
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    • AqT™ Therapeutics
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  • Shop by Brand

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  • Fab preparation kit CM51407
  • Fab preparation kit CM51407

    PerKit™ Fab Preparation Kit

    PerKit™

    MSRP:
    Now: $346.00
    (You save )
    SKU:
    CM51407
    Availability:
    Custom kit made to order, usually ship within 3 days.
    Shipping:
    Calculated at Checkout

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    • Overview

    Product Description

     

    Download Documents
    SDS USER MANUAL CM51407

    In the preparation of antibody-enzyme conjugates there are a few advantages of using antibody fragments rather than intact antibodies. For example, antibody fragments lacking an Fc region have less interference with various Fc binding proteins, can easy penetrate cell membranes due to their lower molecule weight, and experience lower nonspecific binding to cell surfaces. Fab fragments are useful in immuno-histochemical studies.

    CellMosaic has designed this kit for Fab preparation from mouse IgG1 antibody. The procedure we use involves cleaving the IgG molecule in the hinge region above the interchain disulfides using soluble Ficin to create two identical Fab portions and one intact Fc fragment, and then purification to afford the pure Fab.

    This kit provides materials to fragment 0.25-3 mg of mouse IgG1 antibodies with the option to choose from one or three reactions.


     

    Key Features of this PerKit™

    • Easy preparation: 1 day preparation and <2 h hands-on time
    • All reagents and supplies included for preparation and purification
    • 40~60% recovery and 80-90% of pure Fab by SEC (size-exclusion chromatography)
    • Options to choose tailored services at CellMosaic after conjugation

    After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.

     


    Examples

    Figure 1. SEC analysis of a mouse IgG1, crude digestion mixture of the IgG1, and the purified Fab (UV at 220 nm)

    cm51407-figure-1-fab-preparation.png

     

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