CellMosaic’s size exclusion chromatography (SEC) or gel filtration protein standard is designed for day to day use to check HPLC column performance and the analysis of bioconjugates. This product is a new formulation of our previously developed SEC protein standards (Part No. CM92002) and is a replacement for both SEC protein standards (Part No. CM92001 and CM92002). The new formulation allows the stable lyophilization of the protein mixture without changing its HPLC profile. The lyophilized protein mixture can be quickly reconstituted in water without any insoluble particles and is more stable for storage and shippment.
The kit consists of six different proteins and blue dextran with a MW range of 13.7 to 2000 KDa. The broad range of protein molecular weight coverage allows for easy identification of small molecules, conjugates, and aggregates in a bioconjugation mixture (antibody-drug conjugation, protein, and antibody modification and conjugation). This standard has been validated in many of the commonly used SEC columns and CellMosaic routinely uses this product for its internal bioconjugation-related research.
A total of four micro-centrifuge tubes are in a package. Each micro-centrifuge tube contains protein mixture lyophilized from 80 µL of standard solution. The product is ready for use after reconstitution in 80 µL of deionized water.
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Application of the product:
Calibration of gel filtration and size exclusion column
Determination of the size and molecular mass of proteins
Analysis of small molecules, bioconjugates, and aggregates
Key Features of the product:
Lyophilized solid and ready to use for HPLC analysis after dissolving in water
Mixture of 6 high purity proteins with broad MW coverage: 13.7 KDa to 2000 KDa
|3||Ovalbumin (albumin chicken egg)||43|
|4||Aldolase (rabbit muscle)||158|
|5||Ferritin (equine spleen)||440|
Calibration of Protein and Conjugate MW
The approximate MW of an unknown protein or protein conjugate can be determined by comparing its retention time to the protein standard. Figure 1 gives an example of the SEC profile. For more accurate calculation, an SEC MW standard curve is recommended (Figure 2).
Figure 1: SEC HPLC profile of protein standards in a TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, 5 µm, Part No. 0008541). HPLC condition: isocratic PBS buffer at 0.5 mL/min. Instrument: Agilent 1100 HPLC series.
Figure 2: SEC MW standard curve of TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, Part No. 0008541). Data from Figure 1.