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  • SEC HPLC protein standard package
  • SEC (Gel filtration) HPLC Protein Standard (7 components, Lyophilized)
  • SEC HPLC protein standard package SEC (Gel filtration) HPLC Protein Standard (7 components, Lyophilized)

    SEC (Gel filtration) HPLC Protein Standard (7 components, Lyophilized)

    MSRP:
    Now: $177.00
    (You save )
    SKU:
    CM92004
    Availability:
    In stock, usually ships within 24 hrs
    Shipping:
    Calculated at Checkout
    Number of Injections:
    40

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    • Overview

    Product Description

     

    Download Documents
    Product Sheet

    COA-5530.s9.120219

    COA-S216.S18.0715B

    SDS

    Product Description

    CellMosaic’s size exclusion chromatography (SEC) or gel filtration protein standard is designed for day to day use to check HPLC column performance and the analysis of bioconjugates. This product is a new formulation of our previously developed SEC protein standards (Part No. CM92002) and is a replacement for both SEC protein standards (Part No. CM92001 and CM92002). The new formulation allows the stable lyophilization of the protein mixture without changing its HPLC profile. The lyophilized protein mixture can be quickly reconstitution in water without any insoluble particles. The lyophilized product is also more stable for storage and can be shipped at RT.

    The kit consists of six proteins and one blue dextran with a MW range of 13.7 to 2000 KDa. The broad range of protein molecular weight coverage allows easy identification of small molecules, conjugates, and aggregates in a bioconjugation mixture, such as antibody-drug conjugation, protein, and antibody modification and conjugation. The product has been validated in many of the commonly used SEC columns. CellMosaic routinely uses this product for its internal bioconjugation-related research.

    A total of four micro-centrifuge tubes are in a package. Each micro-centrifuge tube contains protein mixture lyophilized from 80 µL of standard solution. The product is ready for use after reconstituting in 80 µL of deionized water.

    Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


    Application of the product:

    • Calibration of gel filtration and size exclusion column
    • Determination of the size and molecular mass of proteins
    • Analysis of small molecules, bioconjugates, and aggregates

    Key Features of the product:

    • Lyophilized solid and ready to use for HPLC analysis after dissolving in water
    • Mixture of 6 high purity proteins with broad MW coverage: 13.7 KDa to 2000 KDa

     Composition

    # Name MW (KDa)
    1     RNase A 13.7
    2     Carbonic anhydrase 29
    3     Ovalbumin (albumin chicken egg) 43
    4     Aldolase (rabbit muscle) 158
    5     Ferritin (equine spleen) 440
    6     Thyroglobulin 669
    7     Blue Dextran 2000

     

     

     

     

     

     

     

     


    Application Note 

    Calibration of Protein and Conjugate MW

    The approximate MW of an unknown protein or protein conjugate can be determined by comparing its retention time to the protein standard. Figure 1 gives an example of the SEC profile. For more accurate calculation, an SEC MW standard curve is recommended (Figure 2). 

    Figure 1: SEC HPLC profile of protein standards in a TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, 5 µm, Part No. 0008541). HPLC condition: isocratic PBS buffer at 0.5 mL/min. Instrument: Agilent 1100 HPLC series. 

    cm92004-sec-proten-standard-figure-1.png

    Figure 2: SEC MW standard curve of TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, Part No. 0008541). Data from Figure 1.

    cm92004-sec-proten-standard-figure-2.png


    Product Videos

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    Number of Injections 40

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