CellMosaic’s size exclusion chromatography (SEC) HPLC LMW protein standard is designed for day to day use to check HPLC column performance and the analysis of bioconjugates. The kit consists of four proteins with a MW range of 6.5 to 43 KDa and a small molecule with MW of 154 Da. The broad range of protein molecular weight coverage allows easy identification of small molecules, conjugates, and aggregates in a bioconjugation mixture, such as protein and peptide labeling and conjugation. CellMosaic routinely uses this product for its internal bioconjugation-related research.
A total of two micro-centrifuge tubes are in a package. Each micro-centrifuge tube contains protein mixture lyophilized from 100 µL of standard solution. The product is ready for use after reconstituting in 100 µL of deionized water.
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Application of the product:
Calibration of gel filtration and size exclusion column
Estimate the size and molecular mass of proteins
Analysis of small molecules, bioconjugates, and aggregates
Key Features of the product:
Lyophilized solid and ready to use for HPLC analysis after dissolving in water
MW coverage: 154 Da to 43 KDa
|5||Ovalbumin (albumin chicken egg)||43,000|
Calibration of Protein and Conjugate MW
The approximate MW of an unknown protein or protein conjugate can be determined by comparing its retention time to the protein standard. Figure 1 gives an example of the SEC profile. For more accurate calculation, an SEC MW standard curve is recommended (Figure 2).
Figure 1: SEC HPLC profile of protein standards in a TSKgel BioAssist G300SWxl (7.8 mm x 30 cm, 5 µm, Part No. 08541). HPLC condition: isocratic PBS buffer at 0.5 mL/min. Instrument: Agilent 1100 HPLC series.
Figure 2: SEC MW standard curve of TSKgel BioAssist G3SWxl (7.8 mm x 300 mm, Part No. 0008541). Data from Figure 1.