Biopolymer Modification
For certain biological applications, it may be necessary to introduce functional groups or linkers onto a biopolymer. The figure on the left illustrates how a biopolymer (M1) reacts with a modification agent (M2) to acquire a functional group (FG). Over the years, CellMosaic has developed a variety of methods to modify biopolymer with unique functional groups for labeling or conjugation, such as aldehyde, keto, maleimide, DBCO, azide, or thiol groups. The introduction of aldehyde groups, for example, allows biopolymer to react selectively at the aldehyde site through reductive amination, oxime formation, or hydrazone formation.
We work with a variety of biopolymers, including antibodies, antibody fragments, enzymes, proteins, peptides, peptidomimetics, DNA, and DNA analogs. For more information on functional group introduction, please click here.
CellMosaic also offers methods for preparing fragmented antibodies, such as Fab, Fab', and bispecific antibody fragments, using a combination of chemical and enzymatic approaches.