This kit is designed to label any IgG antibody with VC-PAB linker. The final labeled product can be used as a negative control for any ADC with VC-PAB linker via thiol labeling chemistry. The preparation is essentially the same as standard ADC preparation with VC-PAB linker. The user supplies the antibody. The kit includes maleimide-activated VC-PAB-OH, which can be coupled directly to the antibody after reduction through alkylation in a single step (a method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The product is purified to remove any unreacted linkers.

This kit provides materials to conjugate 1-3 mg of one (CM11429) or three (CM11429x3) antibody samples (IgG).

If you have less than 1 mg of antibody, please add Antibody PerKit™ Customization for Lower Amount (AS1003) along with this kit. 
If you would like CellMosaic to perform the conjugation instead, please add PerKit™ Custom Conjugation (AS1002) along with this kit.

Shipping policy: Heat sensitive and ship with cold packs, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Linker Information

• Name: Mc-VC-PAB-OH
• CAS number: 159857-80-4
• Chemical formula:  C28H40N6O7
• MW: 572.7 Da

Requirement for Antibody

1. Preferably >90% pure by gel electrophoresis
2. Total amount: 1-3 mg protein content measured by UV

Key Features of this PerKit™

• • Offers a simple and easy way to prepare negative control ADC with cathepsin B cleavable VC-PAB linkage (Ref. Doronina et al. 2008, Bioconjugate Chem. 19, 1960-1963)
• Stable linkage
• Fast and easy preparation: 6 h preparation with <2 h hands-on time
• All reagents and supplies included for preparation and purification
• More than 95% labeled products (free of unreacted linker)
• Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and calculation of the loading.

Conjugation Chemistry

 Protocol

Example of Preparing Control ADC

Antibody information: A therapeutic antibody (human IgG1 subtype)

Kit Lot number: S313.S9.022221

Scale of the reaction: 1.42 mg of antibody (protein content)

Figure 1: HIC HPLC analysis of IgG1 (blue trace) and purified IgG1 labeled with VC-PAB linker (red trace). 

Figure 2. SEC HPLC analysis of IgG1 (blue trace) and purified IgG1 labeled with VC-PAB linker (red trace).

Summary of the results:

LAR: Linker-to-Antibody Ratio (LAR): check the user manual other consideration regarding the LAR calculation.