CellMosaic has designed this kit for antibody (IgG type) labeling of mertansine (DM1) with SMCC (succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate) linker for early stage screening of your antibody.

This kit includes SMCC and DM1, which can be coupled to the antibody sequentially via surface amines (a method developed by Immunogen). The product is purified to remove any unreacted drug. 

This kit provides materials to label either one or three antibodies with the option to choose from three different reaction scales: 3 mg, 1 mg, and 0.1 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Drug Information

• Name: Mertansine (DM1) 
• CAS number: 139504-50-0
• Mechanism of action: Inhibits cell division by blocking the polymerisation of tubulin

Key Features of this PerKit™

• Offers a simple and easy way to label IgG with DM1 with minimum exposure to the toxin
• Stable linkage 
• Fast and easy preparation: 6 h preparation and <2 h hands-on time
• All reagents and supplies included for preparation and purification
• Average 2-4 DM1 labeling per antibody
• Less than 5% of antibody aggregation and >99% of conjugated products by SEC (size-exclusion chromatography)
• Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DOL.

Conjugation Chemistry

Protocol (CM11410 as an example)

Typical Kit Performance Data (LC Analysis, CellMosaic)

Antibody information: A therapeutic antibody (human IgG1 subtype), Kit Lot number: 5513.S9.053018 

Figure 1: SEC HPLC analysis of antibody (blue color) and purified ADC-DM1 (red color) (Inset: UV/Vis spectra of the unlabeled antibody and ADC-DM1.) Scale of the reaction: 1 mg (CM11410.1)

Summary of the result:

R value (A252 nm/A280 nm) of unlabeled antibody: 0.515

The higher the average DAR of ADC-DM1, the greater the percentage of the antibody aggregates and the lower the overall recovery.

Related Products

Maleimide Assay Kit (Cat. CM90002): The average maleimide groups per antibody is 4 after SMCC labeling. If necessary, you can purchase this kit separately from CellMosaic to assay the maleimide content. The kit is simple and easy to use. Approximate total assay time is 1 h.