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SepSphere™ Peptide Immobilization Kit via Maleimide

  • Image 1
  • Schematic diagram of the workflow for peptide immobilization starting with 5 mL of maleimide agarose beads
  • Labeling chemistry
Price:
$549.00
SKU:
CM71301
Brand:
Availability:
In stock, usually ship within 1 week
Shipping:
Calculated at checkout
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SDS USER MANUAL

CellMosaic® has designed this personalized peptide immobilization kit  to work with thiol containing peptide. If your peptide does not have a free thiol for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. The kit provides agarose beads derivatized with maleimide functional groups (5-20 μmol maleimide per mL of resin). Using the kit components, the customer immobilize the thiol containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL


Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Modestly activated maleimide agarose
  • One step immobilization with very mild reaction conditions
  • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
  • Stable linkage (no leakage of the peptides from the resin under acid or base condition)
  • Options to choose tailored custom services at CellMosaic prior to and after conjugation in your lab:

Prior to conjugation, you can supply your peptide information when you place your order and CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation you can choose to send your sample to CellMosaic for HPLC analysis of the peptide samples for determination of the peptide loading.


Specification of the Solid Support

  • Functional groups: maleimide
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 μm 
  • Mean bead size: 90 μm   
  • Ligand concentration: 5-20 μmol maleimide per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via thiol ether bond
  • Chemical stability: stable 
  • pH Stability: stable     

 Requirement for peptide 

  • Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-terminus or N-terminus
  • HPLC purified and lyophilized, stored at -20°C as lyophilized solid 
  • Preferably >90% pure by C18 HPLC
  • Total amount: 1-20 mg 
  • Does not contain any other reducing reagent such as DTT and mercaptoethanol

·         Options to choose tailored custom services at CellMosaic prior to and after conjugation in your lab:

o   Prior to conjugation, you can supply your peptide information when you place your order and CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

o   After conjugation you can choose to send your sample to CellMosaic for HPLC analysis of the peptide samples for determination of the peptide loading.

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