CellMosaic® has designed this personalized peptide immobilization kit  to work with thiol containing peptide. If your peptide does not have a free thiol for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. The kit provides agarose beads derivatized with maleimide functional groups (5-20 μmol maleimide per mL of resin). Using the kit components, the customer immobilize the thiol containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

• Minimum requirement for immobilization set-up
• All reagents, buffers, and plasticwares are included 
• Modestly activated maleimide agarose
• One step immobilization with very mild reaction conditions
• Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
• Stable linkage (no leakage of the peptides from the resin under acid or base condition)
• Options to choose tailored custom services at CellMosaic prior to and after conjugation in your lab:

Prior to conjugation, you can supply your peptide information when you place your order and CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation you can choose to send your sample to CellMosaic for HPLC analysis of the peptide samples for determination of the peptide loading.

Specification of the Solid Support

• Functional groups: maleimide
• Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
• Particle size: 45-165 μm 
• Mean bead size: 90 μm   
• Ligand concentration: 5-15 μmol maleimide per mL agarose

 Specification of the Immobilized Product

• Immobilized chemistry: immobilized via thiol ether bond
• Chemical stability: stable 
• pH Stability: stable     

 Requirement for peptide 

• Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-terminus or N-terminus
• HPLC purified and lyophilized, stored at ≤-20°C as lyophilized solid 
• Preferably >90% pure by C18 HPLC
• Total amount: 1-20 mg 
• Does not contain any other reducing reagent such as DTT and mercaptoethanol

·         Options to choose tailored custom services at CellMosaic prior to and after conjugation in your lab:

o   Prior to conjugation, you can supply your peptide information when you place your order and CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

o   After conjugation you can choose to send your sample to CellMosaic for HPLC analysis of the peptide samples for determination of the peptide loading.