Product Description
| Download Documents (Coming Soon) | |
| SDS | USER MANUAL |
CellMosaic® has designed this oligo immobilization kit to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. The kit provides agarose beads derivatized with epoxide functional groups (~2 μmol epoxide per mL of resin). Using the kit components, the customer immobilize the amino containing oligo onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.
Scale of the reaction: 5 mL
Key Features of this Immobilization Kit:
-
Minimum requirement for immobilization set-up
-
All reagents, buffers, and plasticwares are included
-
Modestly activated epoxide agarose
-
One step immobilization with very mild reaction conditions
-
Easy preparation with air push mechanism for washing: less than 1 h hands-on time
-
Stable linkage
Specification of the Solid Support
- Functional groups: epoxide
- Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
- Particle size: 45-165 um
- Mean bead size: 90 um
- Ligand concentration: ~2 μmol epoxide per mL agarose
Specification of the Immobilized Product
- Immobilized chemistry: immobilized via stable ether bond
- pH Stability: stable
Requirement for oligo
- Preferable 5' C6 or 3' C6 amino modified oligo
- HPLC purified and lyophilized
- Total amount: 0.1-1 μmol
- Additional components of acid or other molecules containing primary or secondary amine groups and thiol should NOT be present
Potential interfering compounds for labeling and conjugation reactions:
a. Thiols: e.g., DTT and mercaptoethanol
b. Primary and secondary amines: e.g., ammonium acetate, ammonium bicarbonate, ammonium tartrate, ammonium citrate, ammonium phosphate, AMPD [2-amino-2-methyl-1,3 propanediol], aminoguanidine bicarbonate salt, ethanol amine, Gly-gly, Tris buffer, piperidine buffers.
