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SepSphere™ Oligo Immobilization Kit via Epoxide

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CellMosaic® has designed this oligo immobilization kit to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. The kit provides agarose beads derivatized with epoxide functional groups (~2 μmol epoxide per mL of resin). Using the kit components, the customer immobilize the amino containing oligo onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Modestly activated epoxide agarose
  • One step immobilization with very mild reaction conditions
  • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
  • Stable linkage 

Specification of the Solid Support

  • Functional groups: epoxide
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: ~2 μmol epoxide per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via stable ether bond
  • pH Stability: stable     

 Requirement for oligo 

  • Preferable 5' C6 or 3' C6 amino modified oligo
  • HPLC purified and lyophilized
  • Total amount: 0.1-1 μmol 
  • Additional components of acid or other molecules containing primary or secondary amine groups and thiol should NOT be present

Potential interfering compounds for labeling and conjugation reactions:
a. Thiols: e.g., DTT and mercaptoethanol
b. Primary and secondary amines: e.g., ammonium acetate, ammonium bicarbonate, ammonium tartrate, ammonium citrate, ammonium phosphate, AMPD [2-amino-2-methyl-1,3 propanediol], aminoguanidine bicarbonate salt, ethanol amine, Gly-gly, Tris buffer, piperidine buffers.

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