Download Documents | |
SDS | USER MANUAL |
CellMosaic® has designed this oligo immobilization kit to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. The kit provides agarose beads derivatized with epoxide functional groups (~2 μmol epoxide per mL of resin). Using the kit components, the customer immobilize the amino containing oligo onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.
Scale of the reaction: 5 mL
Key Features of this Immobilization Kit:
Specification of the Solid Support
Specification of the Immobilized Product
Requirement for oligo
Potential interfering compounds for labeling and conjugation reactions:
a. Thiols: e.g., DTT and mercaptoethanol
b. Primary and secondary amines: e.g., ammonium acetate, ammonium bicarbonate, ammonium tartrate, ammonium citrate, ammonium phosphate, AMPD [2-amino-2-methyl-1,3 propanediol], aminoguanidine bicarbonate salt, ethanol amine, Gly-gly, Tris buffer, piperidine buffers.