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SDS | USER MANUAL |
CellMosaic® has designed this personalized peptide immobilization kit to work with peptide containing amino functional groups. All natural peptide has an N-terminal amino group that can react with carboxylic acid groups. If you have Lysine amino acids, the ε-amino group of lysine may also react. If you prefer a site specific single labeling strategy, please check your peptide sequence to make sure only one amino groups present in the peptide. N-terminal amino group can be acetylated during the SPPS to make it inert. You can also add a Lys group in the peptide to position your peptide on the agarose beads if you don't have any amino group.
The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, the customer immobilize the amino containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.
Scale of the reaction: 5 mL
Key Features of this Immobilization Kit:
Specification of the Solid Support
Specification of the Immobilized Product
Requirement for peptide