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SepSphere™ Peptide Immobilization Kit via Carboxylic Acid

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CellMosaic® has designed this personalized peptide immobilization kit to work with peptide containing amino functional groups. All natural peptide has an N-terminal amino group that can react with carboxylic acid groups. If you have Lysine amino acids, the ε-amino group of lysine may also react. If you prefer a site specific single labeling strategy, please check your peptide sequence to make sure only one amino groups present in the peptide. N-terminal amino group can be acetylated during the SPPS to make it inert. You can also add a Lys group in the peptide to position your peptide on the agarose beads if you don't have any amino group.

The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, the customer immobilize the amino containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Modestly activated carboxylic acid agarose
  • One step immobilization with very mild reaction conditions
  • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
  • Stable linkage (no leakage of the peptides from the resin under acid or base condition)

Specification of the Solid Support

  • Functional groups: carboxylic acid (COOH)
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: 5-20 μmol COOH per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via stable amide bond
  • Chemical stability: stable 
  • pH Stability: stable     

 Requirement for peptide 

  • Have at least one amino functional group in the peptide (Note: multiple amino groups in a peptide can result in a mixture of immobilized products at various positions). Amino groups in peptide that can react with carboxylic acid 
    • N-terminal amino group if it is not acetylated or blocked 
    • ε-amino group of lysine
  • HPLC purified and lyophilized
  • Preferably >90% pure by C18 HPLC
  • Total amount: 1-20 mg 

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