Product Description
CellMosaic® has designed this personalized immobilization kit for small molecule containing amine functional group. The user supplies the small molecule amine. Using the kit components, the customer immobilizes the small molecule amine onto SepSphere™ carboxylic acid agarose in one step following CellMosaic® standard protocol.
This kit is custom made to order.
Some small molecules may contain other functional groups that can interfere with the reaction. We recommend that customers first email information about their small molecule before placing an order for this kit so we can provide insight into the efficacy of this kit and potentially present alternative options.
Key Features of this Immobilization Kit:
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Minimum requirement for immobilization set-up
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All reagents, buffers, and plasticwares are included
- Mild reaction conditions
- Stable attachment
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Easy preparation with air push mechanism for washing: less than 2 hr hands-on time
Specification of the Solid Support
- Functional groups: carboxylic acid
- Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
- Particle size: 45-165 um
- Mean bead size: 90 um
- Ligand concentration: 5-20 μmol carboxylic acid per mL agarose
Specification of the Immobilized Product
- Immobilized chemistry: immobilized via amide bond formation
- Chemical stability: stable
- pH Stability: stable
Requirement for Small Molecule
- Preferably >90% pure by C18 HPLC
- Total amount: 1-10 mg
