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SepSphere™ Small Molecule Amine Immobilization Kit via Carboxylic Acid

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CellMosaic® has designed this personalized immobilization kit for small molecule containing amine functional group. This kit is custom made to order. Some of the small molecules may contain other functional groups that can interfere with the reaction. We recommend the customer to email your information of your small molecule before placing this order.  

The user supplies the small molecule amine. Using the kit components, the customer immobilizes the small molecule amine onto SepSphere™ carboxylic acid agarose in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Mild reaction conditions
  • Stable attachment
  • Easy preparation with air push mechanism for washing: less than 2 h hands-on time

 Specification of the Solid Support

  • Functional groups: carboxylic acid
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: 5-20 μmol carboxylic acid per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via amide bond formation
  • Chemical stability: stable 
  • pH Stability: stable     

 Requirement for Small Molecule

  • Preferably >90% pure by C18 HPLC
  • Total amount: 1-10 mg 

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