CellMosaic® has designed this personalized peptide immobilization kit to work with thiol peptide. If your peptide does not have a Cys thiol group for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. The kit provides agarose beads derivatized with pyridyl disulfide functional groups (5-20 μmol pyridyl disulfide per mL of resin). Using the kit components, the customer immobilize the thiol containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

• Minimum requirement for immobilization set-up
• All reagents, buffers, and plasticwares are included 
• Modestly activated pyridyl disulfide agarose
• One step immobilization with very mild reaction conditions
• Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
• Stable linkage (no leakage of the peptides from the resin under acid or base condition)

Specification of the Solid Support

• Functional groups: pyridyl disulfide
• Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
• Particle size: 45-165 um 
• Mean bead size: 90 um   
• Ligand concentration: 5-20 μmol pyridyl disulfide per mL agarose

 Specification of the Immobilized Product

• Immobilized chemistry: immobilized via disulfide bond
• Chemical stability: stable but can be cleaved to release the peptide by reducing reagent such as DTT, TCEP
• pH Stability: stable     

 Requirement for peptide 

• Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-termini or N-termini
• HPLC purified and lyophilized, stored at -20 degree freezer all the time
• Preferably >90% pure by C18 HPLC
• Total amount: 1-20 mg 
• Does not contain any other reducing reagent such as DTT and mercaptoethanol