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SepSphere™ Peptide Immobilization Kit via Pyridyl Disulfide

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CellMosaic® has designed this personalized peptide immobilization kit to work with thiol peptide. If your peptide does not have a Cys thiol group for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. The kit provides agarose beads derivatized with pyridyl disulfide functional groups (5-20 μmol pyridyl disulfide per mL of resin). Using the kit components, the customer immobilize the thiol containing peptide onto SepSphere™ agarose beads in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Modestly activated pyridyl disulfide agarose
  • One step immobilization with very mild reaction conditions
  • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
  • Stable linkage (no leakage of the peptides from the resin under acid or base condition)

Specification of the Solid Support

  • Functional groups: pyridyl disulfide
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: 5-20 μmol pyridyl disulfide per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via disulfide bond
  • Chemical stability: stable but can be cleaved to release the peptide by reducing reagent such as DTT, TCEP
  • pH Stability: stable     

 Requirement for peptide 

  • Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-termini or N-termini
  • HPLC purified and lyophilized, stored at -20 degree freezer all the time
  • Preferably >90% pure by C18 HPLC
  • Total amount: 1-20 mg 
  • Does not contain any other reducing reagent such as DTT and mercaptoethanol

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