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SepSphere™ Small Molecule Alcohol Immobilization Kit

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CellMosaic® has designed this personalized immobilization kit for small molecule containing OH functional group. Some of the small molecules may contain other functional groups that can interfere with the reaction. We recommend the customer to email your information of your small molecule before placing this order.  

The kit is designed to work with small molecules containing one hydroxyl group. The customer supplies the small molecule. Using the kit components, the customer converts the hydroxyl group of the small molecule to an activated chloroacetyl group and the amine-modified agarose beads to a thiol-modified agarose, followed by the reaction of both components. Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Offers a simple and easy way to label small molecules with a hydroxyl group
  • Includes all reagents and supplies needed for preparation
  • Uses agarose beads with an optimized amount of amine functional groups for loading to preserve the nature of the agarose beads
  • Minimum requirement for immobilization set-up
  • Easy preparation with air push mechanism for washing: 6 h preparation time and less than 2 h hands-on time

 Specification of the Solid Support

  • Functional groups: amine 
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: 10-30 umole per ml of settle beads

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via amino agarose
  • Chemical stability: all commonly used aqueous buffers
  • pH Stability: long term: 3 to 9; short term: 2 to 10
  • Physical stability: negligible volume variation due to changes in pH or ionic strength    

 Requirement for Small Molecules

  • Preferably >90% pure 
  • Absence of other reactive functional groups, such as amine and thiol groups 
  • For molecules containing a phenol group, multiple hydroxyl groups, or a secondary hydroxy group, please consult CellMosaic prior to conducting the experiment    

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