CellMosaic® has designed this personalized immobilization kit for small molecule containing ketone or aldehyde functional group. Some of the small molecules may contain other functional groups that can interfere with the reaction. We recommend the customer to email your information of your small molecule before placing this order.  

The user supplies the small molecule ketone. Using the kit components, the customer immobilizes the small molecule onto SepSphere™ aminooxy agarose in one step following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

• Offers a simple and easy way to label small molecules with ketone or aldehyde groups
• Includes all reagents and supplies needed for preparation
• Uses agarose beads with an optimized amount of aminooxy functional groups for loading to preserve the nature of the agarose beads
• Minimum requirement for immobilization set-up
• Easy preparation with air push mechanism for washing: 6 h preparation time and less than 2 h hands-on time

 Specification of the Solid Support

• Functional groups: aminooxy
• Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
• Particle size: 45-165 um 
• Mean bead size: 90 um   
• Ligand concentration: 5-10 μmol aminooxy per mL agarose

 Specification of the Immobilized Product

• Immobilized chemistry: immobilized via oxime bond
• Chemical stability: stable 
• pH Stability: stable (2-10)  

 Requirement for Small Molecule

• Preferably >90% pure by C18 HPLC
• Total amount: 1-10 mg 
• Stable under slightly acidic aqueous conditions