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SepSphere™ Oligo Immobilization Kit for Phosphate DNA

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CellMosaic® has designed this oligo immobilization kit to work with oligo containing a 5' phosphate group. For PCR product you can order a primer with a 5'-phosphate group or use polynucleotide kinase to put the phosphate at the end. You can also order phosphate containing oligo directly from many commercial oligo suppliers. The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, the customer immobilize the phosphate containing oligo onto SepSphere™ agarose beads in two steps following CellMosaic® standard protocol.

Scale of the reaction: 5 mL

Key Features of this Immobilization Kit:

  • Minimum requirement for immobilization set-up
  • All reagents, buffers, and plasticwares are included 
  • Modestly activated carboxylic acid agarose
  • One step immobilization with very mild reaction conditions
  • Easy preparation with air push mechanism for washing: less than 1 h hands-on time 
  • Stable linkage

Specification of the Solid Support

  • Functional groups: COOH
  • Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
  • Particle size: 45-165 um 
  • Mean bead size: 90 um   
  • Ligand concentration: 5-20 μmol COOH per mL agarose

 Specification of the Immobilized Product

  • Immobilized chemistry: immobilized via stable amide bond
  • Chemical stability: stable 
  • pH Stability: stable     

 Requirement for oligo 

  • >90% by gel electrophoresis 
  • Total amount: 0.1-2 μmol 
  • >10 nucleotide bases

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