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Antibody MMAE Conjugation Kit (With VC-PAB Linkage)

  • Image 1
  • CM11409 MC-VC-PAB MMAE chemical structure
Price:
$990.00
SKU:
CM11409
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Quantity:


 

Download Documents
SDS USER MANUAL CM11409
USER MANUAL CM11409.1 USER MANUAL CM11409.01

CellMosaic has designed this kit for labeling any IgG1 antibody with monomethyl auristatin E (MMAE) using a valine-citruline p-aminobenzylcarbamate (VC-PAB) linker for early stage screening of your antibody. 

This kit includes maleimide-activated VC-PAB-MMAE, which can be coupled directly to the IgG1 after reduction through alkylation in a single step (a method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The product is purified to remove any unreacted drug. 

Kit is configured to label either one or three antibodies with the option to choose from three different reaction scales: 3 mg, 1 mg, and 0.1 mg

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Drug Information

  • Name: Monomethyl auristatin E (MMAE) with Mal-Val-Cit-PAB linkage
  • CAS number: 646502-53-6
  • Mechanism of action: Inhibits cell division by blocking the polymerization of tubulin. VC-PAB linker is stable in extracellular fluid, but cleaved by cathepsin B once inside the tumor cell, thus activating the antimitotic mechanism
  • Activities: Antioxidant, anti-inflammatory, anticancer, and insecticidal activities

Requirement for Antibody

  1. IgG1 subtype only (The kit protocol is optimized for IgG1 subtype. If you have other IgG subtypes, please contact CellMosaic for customization.)
  2. Preferably >90% pure by gel electrophoresis
  3. Total amount: 0.1 mg for CM11410.01, 1 mg for CM11410.1, and 3 mg for CM11410 (protein content measured by UV. If you don't have the exact amount, please contact CellMosaic for customization.

Key Features of this PerKit™

  • Offers a simple and easy way to label IgG1 with MMAE with minimum exposure to the toxin
  • Cathepsin B cleavable VC-PAB (Ref. Doronina et. al. 2008, Bioconjugate Chem. 19, 1960-1963)
  • Fast and easy preparation: 6 h preparation and <2 h hands-on time
  • All reagents and supplies included for preparation and purification
  • Over 95% conjugated products (free of unreacted MMAE and less than 5% of unreacted antibody)
  • Options to choose tailored services at CellMosaic after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis and determination of the DOL.

 


Conjugation Chemistry

cm11409-antibody-mmae-conjugation-chemistry.png


Protocol (CM11409 as an example)

cm11409-antibody-mmae-kit-protocol.png

 


Related Products

Thiol Assay Kit (Cat. CM90004)The average free thiol groups per antibody is 4 after reducing. If necessary, you can purchase this kit separately from CellMosaic to measure the free thiols. Total assay time is 20 minutes.

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