MMAE (monomethyl auristatin E) is an antimitotic agent that binds to tubulin and inhibits the assembly of microtubules. Mc-VC-PAB-MMAE is a thiol reactive derivative of MMAE that containing a cathepsin cleavable valine-citruline p-aminobenzylcarbamate linkage (VC-PAB). Mc-VC-PAB-MMAE is used to prepare antibody-drug conjugates, such as brentuximab vedotin (Seattle Genetics).

This product is a control ADC for any IgG antibody labeled with MMAE, in particularly ADC made using CellMosaic's ADC Kits (Cat#: CM11409). The product uses a human IgG1 subtype antibody that does not bind to the target. It has an average 4 MMAE loaded on each antibody and can be used for accessing the effect of MMAE alone (non-specific toxicity). The product uses a very low endotoxin level antibody as a starting material (≤0.5 EU/mg by the LAL method). The preparation and purification are conducted under pyrogen free environment. The final product is assayed for endotoxin to make sure it is suitable for cell culture and animal studies. The loading is confirmed by various methods including UV, HPLC, and difference MALDI-TOF MS.

The final conjugate is lyophilized from phosphate buffered saline containing sugar-based stabilizer for easy shipping and storage. 

The product is sold as 1 vial of 100 microgram (Cat# CM51100-100UG), 1 vial of 500 microgram (Cat# CM51100-500UG), or 2 vials of 500 microgram (Cat# CM51100-1MG). For bulk orders, please contact us for a quote.

Chemical Information

• Chemical name: ADC Control hIgG1-MMAE
• Chemical formula: N/A
• Molecular weight: 152KDa           CAS number: N/A

 Specification (current COA)

• Physical appearance: Colorless to white lyophilized powder in a microcentrifuge tube
• Storage temp.: -20°C
• Purity: ≥99% of MMAE labeled antibody by SEC HPLC, free of any unreacted MMAE
• Endotoxin Level: ≤0.5 EU/mg by the LAL method
• Drug over antibody ratio (DAR): ~4 by UV ratio, HIC HPLC, and difference MALDI-TOF MS

Antibody Information

• Immunogen: HEL (Hen Egg Lysozyme) 
• Species Reactivity: chicken
• Expression Host: CHO-S cell line
• Class: Human IgG1, Kappa
• Antibody Type: Monoclonal
• Applications: ELISA, FACS, IF, IHC, in vivo studies, IP, WB
• Purification Method: Affinity purified

Related Products

Antibody MMAE Conjugation Kit (With VC-PAB Linkage) (Product #: CM11409): ADC kit for labeling any IgG antibody with MMAE using a VC-PAB linker for early stage screening of customer's antibody.