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SDS | COA_Lot#NB0350512 |
CellMosaic’s Phenyldiazirine sxLink™ (T2A14) is a proprietary photo-crosslinking reagent developed at CellMosaic for studying biomolecular interactions. This reagent combines the highly efficient carbene-generating phenyldiazirine group with a cleavable disulfide crosslinking group via a super hydrophilic AqueaTether™ (AqT™) based linker. These structural features make it highly advantageous to use this reagent over traditional photo-crosslinking reagents.
The product is sold as 1 vial of 250 µg (Cat# CM81401-250UG) or 4 vials of 250 µg (Cat# CM81401-4x250UG) lyophilized powder. One vial of dissolution buffer is included
Chemical Information
Specification
Application of the Product
Key Features of the Product
Figure 1. sxLink™ workflow using water-soluble Phenyldiazirine sxLink™ (T2A14)
Figure 2. C18 HPLC analysis of 4-[3-(trifluoromethyl)-3H-diazirin-3-yl]benzoic acid (TFDB, red, in DMSO) and sxLink™ (blue, in water).
References
Protein crosslinking reviews: a) Brunner, J. (1993) Annu. Rev. Biochem. 62, 485−514. b) Freedman, R. B. (1979) Trends Biochem. Sci. 193–197. c) Herrmann, J. M., Westermann, B., Neupert, W. (2001) Methods Cell Biol. 65, 217–230. d) Fancy, D. A. (2000) Curr. Opin. Chem. Biol. 4, 28–32. e) Fasold, H., Klappenberger, J., Meyer, C., Remold, H. (1971) Angew. Chem. Internat. Edit. 10, 795–801. f) Bayley, H. Photogenerated Reagents in Biochemistry and Molecular Biology, Vol. 12. Elsevier, Amsterdam, Neth, 1983.
3-Trifluoromethyl-3-phenyldiazirine reference: Brunner, J., Senn, H. & Richards, F. M. (1980) J. Bio. Chem. 255, 3313−3318.
Thiol cleavable photo-crosslinking reagents for studying rhodopsin and transducing interactions: a) Resek, J. F., Bhattacharya, S. & Khorana, H. G. (1993) J. Org. Chem. 58, 7598−7601. b) Resek, J. F., Farrens, D. & Khorana, H. G. (1994) Proc. Natl. Acad. Sci. USA 91, 7643−7647. c) Cai, K, Itoh, Y. & Khorana, H. G. (2001) Proc. Natl. Acad. Sci. USA 98, 4877−4882. d) Huang Y, Khorana HG. (2003) Mapping of Contact Sites in Interaction between Transducin and Light-Activated Rhodopsin. Presented at 17th Symposium of the Protein Society, July 26–30, Boston, Massachusetts.