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Fab—HRP conjugate

  • Image 1
  • Scheme: thiol modified Fab reaction with maleimide activated enzyme
Price:
The standard price is $2300 for 3 mg of starting biopolymers. Please contact us for a quotation.
SKU:
BC0004


Product Description

  • Chemistry: 2-iminothiolane modified Fab conjugate reaction with  Maleimide activated enzyme HRP.
  • Specification: A pool of heterogeneous products.
  • Procedure: After a buffer exchange (if necessary), labeling of HRP (the enzyme) with NHS ester maleimide followed by gel filtration to remove excess crosslinking reagents. Then thiolation of Fab by 2-iminothiolane, conjugation of Fab to the labeled HRP and purification. Finally, concentration determination and gel electrophoresis analysis of the Fab-HRP conjugates.
  • Price: Standard price covers labor and materials including cost of HRP and its labeling, Fab thiolation, conjugation procedure, conjugate purification, and analysis.
  • Starting materials: Please check biopolymer for ordering information.
  • Note: In this case sulfhydryl functional groups will be introduced to Fab through Lys residues using a reliable and reproducible procedure. However, if some of the modified or conjugated Lys residues are located on the antigen binding sites, this method may produce partially active or inactive Fab-enzyme conjugates that may not bind to the antigen. In such a case an alternative conjugation method may be used.
  • Discount: 15 % discount price applies to addtional conjugates ordered at the same time.
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