The kit is designed to work with antibody IgG. The customer supplies its own unmodified IgG. Using the kit components, the customer converts their antibody to a thiol-antibody, followed by reaction of the thiol-antibody with activated HRP to generate the HRP-antibody conjugates. One step purification typically provides the resulting HRP-antibody at greater than 95% purity.

This kit provides materials to label one (CM51406x1) to three antibody samples (CM51406x3). Total amount of activated HRP included in a reaction: 2 mg. 

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Requirement for Antibody:

• IgG type (for other antibody type, please contact us for a custom bioconjugation)
• Amount: 1.2 mg per reaction (Lower amount of IgG is fine, but need customization. Please contact us for the customization)
• Preferably >90% pure by gel electrophoresis or SEC HPLC analysis

Key Features of this HRP-Antibody Conjugation Kit:

• High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
• Target 1-3 HRP per antibody for a typical batch
• A single purification step affords over 95% pure of HRP-antibody conjugates 
• Fast preparation: less than 3 h hands-on time
• All reagents included, from preparation to purification
• Options to choose tailored services at CellMosaic after conjugation

After conjugation, you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.

 Labeling Chemistry