The kit is designed to work with antibody IgG. The customer supplies its own unmodified IgG. Using the kit components, the customer converts their antibody to a thiol-antibody, followed by reaction of the thiol-antibody with activated HRP to generate the HRP-antibody conjugates. One step purification typically provides the resulting HRP-antibody at greater than 95% purity.
This kit provides materials to label one (CM51406x1) to three antibody samples (CM51406x3). Total amount of activated HRP included in a reaction: 2 mg.
Requirement for Antibody:
- IgG type (for other antibody type, please contact us for a custom bioconjugation)
- Amount: 1.2 mg per reaction (Lower amount of IgG is fine, but need customization. Please contact us for the customization)
- Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Key Features of this HRP-Antibody Conjugation Kit:
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Target 1-3 HRP per antibody for a typical batch
A single purification step affords over 95% pure of HRP-antibody conjugates
Fast preparation: less than 3 h hands-on time
All reagents included, from preparation to purification
- Options to choose tailored services at CellMosaic after conjugation
After conjugation, you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.