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SDS | USER MANUAL |
CellMosaic® has designed this personalized HRP–protein conjugation kit to work with any protein with a MW over 20KDa. For protein with a MW less than 20KDa, please check CM52415 instead. The customer supplies its own unmodified protein. Using the kit components, the customer converts some of the surface amines of the protein to free thiol groups, followed by reaction of the thiol-protein with activated HRP to generate the HRP–protein conjugates. If there are any unreacted thiols on the protein, they are subsequently capped. One step purification typically provides the resulting HRP–Protein at greater than 90% content.
This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.
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Requirement for protein:
Key Features of this HRP-Protein Conjugation Kit:
Labeling Chemistry