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    Main Menu

  • About Us
    • Our History
    • Our Mission
    • Our Approach
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  • Products and Services
  • How to Order
    • Ordering Products
      • Terms and Conditions for Sales of Products
    • Ordering Services
    • Shipping & Returns
    • Distributors
  • Technologies
    • AqueaTether™ (AqT™) Technologies
    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
    • NeIon™ Technologies
  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
      • Biopolymer Supply
    • Frequently Asked Questions
    • Introducing Functional Groups
    • Downloadable Documents
  • Careers
  • Shop By Category

  • Antibody-Drug Conjugate (ADC) Kits and Solution
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    • Routine ADC Synthesis
    • ADC Controls
    • Buffers and others
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  • Personalized Conjugation Kits (PerKit™)
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    • Peptide Labeling and Conjugation Kits
    • Protein Labeling and Conjugation Kits
    • Enzyme Labeling and Conjugation Kits
    • Small Molecule Labeling and Conjugation Kits
    • Antibody Labeling and Conjugation Kits
    • Antibody Fragmentation Kits
    • Antibody Production and Immunoassay Tools
    • Biotin and Streptavidin Labeling
    • Others
  • Standards and Reagents
    • Analytical Standards
    • Assay Kits
    • Drug Intermediates and Metabolites
    • Peptides and Peptide Conjugates
    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
  • Routine Bioconjugation
    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
    • Antibody Drug Conjugates (ADC) Solutions
    • Bioconjugate Drug Development
    • Diagnostic Conjugates Development & Manufacturing
    • Biologics, Bioconjugate Analysis and Purification
    • Other Services
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    • AqT™ Therapeutics
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  • HRP Protein Conjugate HMW
  • HRP-Protein Conjugation Kit (HMW)
  • HRP Protein Conjugate HMW HRP-Protein Conjugation Kit (HMW)

    HRP-Protein Conjugation Kit (HMW)

    PerKit™

    Contact us for a quote for custom synthesis.

    (You save )
    SKU:
    CM52414
    Availability:
    Conjugate preparation at CellMosaic due to the complexities of the biomolecules.

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    • Overview

    Product Description

     

    Download Documents
    SDS USER MANUAL

    CellMosaic® has designed this personalized HRP–protein conjugation kit to work with any protein with a MW over 20KDa. For protein with a MW less than 20KDa, please check CM52415 instead. The customer supplies its own unmodified protein. Using the kit components, the customer converts some of the surface amines of the protein to free thiol groups, followed by reaction of the thiol-protein with activated HRP to generate the HRP–protein conjugates. If there are any unreacted thiols on the protein, they are subsequently capped. One step purification typically provides the resulting HRP–Protein at greater than 90% content.

    This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.


    Requirement for protein:

    • Amount: 8 nmol (400 µg if the MW of protein is 50KDa) (Lower amount of protein is fine, but need customization. Please contact us for the customization)
    • Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
    • Protein has some lysine residues

    Key Features of this HRP-Protein Conjugation Kit:

    • Use of heterobifunctional crosslinkers for direct labeling and conjugation in a two-step process
      • Better control over the conjugation process,
      • Limit self coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
    • High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
    • Target 1−3 HRP per protein (actual loading will depend on your protein)
    • ≥90% HRP−Protein conjugates if the quality of the protein is at par 
    • Preparation can be done in a day with less than 3 h hands-on time
    • All reagents included, from preparation to purification
    • Options to choose tailored services at CellMosaic after conjugation
      • You can choose to send your conjugates to CellMosaic for HPLC analysis of the sample or complete removal of HRP and/or unreacted protein.

     Labeling Chemistry

     scheme-1-hrp-protein-hmw.png

     

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