HRP-Protein Conjugation Kit (LMW)

Product Description

CellMosaic® has designed this personalized conjugation kit to work with any small protein with a MW less than 20 KDa. For protein with a MW over 20 KDa, please check CM52414 instead. Customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a thiol-protein, followed by reaction of the thiol-protein with activated HRP to generate the HRP-protein conjugates. The purification process typically provides the resulting HRP-protein at greater than 95% purity.

This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.

Requirement for protein:

• Amount: 230 nmol (3.45 mg if the protein is 15 KDa) (Lower amount of protein is fine, but need customization. Please contact us for the customization)
• Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
• Protein has some lysine residues

Key Features of this HRP-Protein Conjugation Kit:

• Use of heterobifunctional crosslinkers for direct labeling and conjugation in a two-step process
• Better control over the conjugation process,
• Limit self coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
• High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
• Optimal maleimide groups per HRP for single label
• Target 1-2 protein per HRP for a typical batch
• A single purification step affords over 95% pure of HRP-protein conjugates 
• Fast preparation: less than 3 h hands-on time
• All reagents included, from preparation to purification
• Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your protein information when you place your order. CellMosaic will give recommendations for the conjugation if your protein has special features.

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.

 Labeling Chemistry