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  • About Us
    • Our History
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  • Products and Services
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      • Terms and Conditions for Sales of Products
    • Ordering Services
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  • Technologies
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    • oxLink™ and sxLink™ Photocrosslinking Technologies
    • Advanced Conjugation Processes
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  • Resources
    • Custom Conjugation Guide
      • Small Molecule Supply
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    • Others
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    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
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    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
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    • Bioconjugate Drug Development
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    • Biologics, Bioconjugate Analysis and Purification
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  • HRP Protein LMW Conjugate
  • HRP Protein LMW Conjugation Scheme
  • HRP Protein LMW Conjugate HRP Protein LMW Conjugation Scheme

    HRP-Protein Conjugation Kit (LMW)

    PerKit™

    Contact us for a quote for conjugation using this kit

    (You save )
    SKU:
    CM52415
    Availability:
    Conjugate preparation at CellMosaic due to the complexities of the biomolecules.

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    Product Description

     

    Download Documents
    SDS USER MANUAL

    CellMosaic® has designed this personalized conjugation kit to work with any small protein with a MW less than 20 KDa. For protein with a MW over 20 KDa, please check CM52414 instead. Customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a thiol-protein, followed by reaction of the thiol-protein with activated HRP to generate the HRP-protein conjugates. The purification process typically provides the resulting HRP-protein at greater than 95% purity.

    This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.


    Requirement for protein:

    • Amount: 230 nmol (3.45 mg if the protein is 15 KDa) (Lower amount of protein is fine, but need customization. Please contact us for the customization)
    • Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
    • Protein has some lysine residues

    Key Features of this HRP-Protein Conjugation Kit:

    • Use of heterobifunctional crosslinkers for direct labeling and conjugation in a two-step process
      • Better control over the conjugation process,
      • Limit self coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
    • High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity 
    • Optimal maleimide groups per HRP for single label
    • Target 1-2 protein per HRP for a typical batch
    • A single purification step affords over 95% pure of HRP-protein conjugates 
    • Fast preparation: less than 3 h hands-on time
    • All reagents included, from preparation to purification
    • Options to choose tailored services at CellMosaic prior to and after conjugation

    Prior to conjugation: you can supply your protein information when you place your order. CellMosaic will give recommendations for the conjugation if your protein has special features.

    After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.


     Labeling Chemistry

     cm52414-hrp-protein-hmw-scheme.png

     

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