Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, and multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

This kit provides materials to perform one KLH labeling. Total amount of KLH included: 5 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Requirement for Small Molecule

• Preferably > 90% pure
• Absence of primary or secondary amine (-NH2) groups
• Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
• For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic first before placing the order.

Key Features of this PerKit™

• Offers a simple and easy way to label KLH with any small molecule containing carboxylic acid
• Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
• All reagents and supplies included for preparation and purification
• Over 90% pure conjugated products by SEC
• Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will general a PerKit™ sheet containing calculation, chemical structure, MW of your final conjugate, and recommendations for the conjugation if your molecule has special features. 

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.

Protocol

Labeling Chemistry