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PerKit™ KLH Universal Small Molecule Acid Conjugation Kit

  • KLH Small Acid Conjugates
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Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, and multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated Sulfo N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

This kit provides materials to perform one KLH labeling. Total amount of KLH included: 5 mg.

Requirement for Small Molecule

  • Preferably contains only one carboxylic acid (-COOH)
  • Preferably > 90% pure
  • Absence of primary or secondary amine (-NH2) groups
  • The presence of the hydroxide (-OH) group will not affect the labeling

Key Features of this PerKit™

  • Offers a simple and easy way to label KLH with any small molecule containing carboxylic acid
  • Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
  • All reagents and supplies included for preparation and purification
  • Over 90% pure conjugated products by SEC
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will check if your molecule is compatible with this kit. 

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.


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