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SDS | USER MANUAL |
Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, and multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated Sulfo N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid.
This kit provides materials to perform one KLH labeling. Total amount of KLH included: 5 mg.
Requirement for Small Molecule
Key Features of this PerKit™
Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic will check if your molecule is compatible with this kit.
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis.