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    • Peptide and Protein
    • Kits
    • PerKit® KLH Small Molecule Acid Conjugation Kit
  • Chemical structure of KLH Small Acid Conjugates generated using PerKit KLH Small Molecule Acid Conjugation Kit
  • Chemical structure of KLH Small Acid Conjugates generated using PerKit KLH Small Molecule Acid Conjugation Kit

    PerKit® KLH Small Molecule Acid Conjugation Kit

    PerKit®

    MSRP:
    Now: $706.00
    (You save )
    SKU:
    CM52412
    Availability:
    Custom kit made to order, usually ship within 1 week.
    Shipping:
    Calculated at Checkout

    Maximum file size is 200, file types are bmp, gif, jpg, jpeg, jpe, jif, jfif, jfi, png, wbmp, xbm, tiff, cdx, pdf

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    • Overview

    Product Description

    Download Documents
    SDS USER MANUAL

    Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, and multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any small molecule containing a carboxylic acid (-COOH) functional group. Using the kit components, the customer converts the carboxylic acid to an activated N-hydroxysuccinimide (NHS) ester, followed by reaction with the surface amino groups of the protein to form a stable amide bond. The final product is purified to remove any unreacted small molecule acid. 

    This kit provides materials to perform one KLH labeling. Total amount of KLH included: 5 mg.


    Requirement for Small Molecule

    • Preferably > 90% pure
    • Absence of primary or secondary amine (-NH2) groups
    • Preferably contains only one non-hindered aliphatic carboxylic acid (-COOH)
    • For molecule containing aromatic carboxylic acid, hindered aliphatic carboxylic acid, or hydroxyl (-OH) groups, please consult CellMosaic® first before placing the order.

    Key Features of this PerKit™

    • Offers a simple and easy way to label KLH with any small molecule containing carboxylic acid
    • Fast and easy preparation: 4 h preparation and <30 minutes hands-on time
    • All reagents and supplies included for preparation and purification
    • Over 90% pure conjugated products by SEC
    • Options to choose tailored services at CellMosaic® prior to and after conjugation

    Prior to conjugation: you can upload your small molecule information when you place your order. CellMosaic® will check if your molecule is compatible with this kit. 

    After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis.


    Protocol Diagram

    Reaction Scheme/Workflow for PerKit™ KLH Small Molecule Acid Conjugation Kit

     


    Labeling Chemistry

    Labeling chemistry used in PerKit™ KLH Small Molecule Acid Conjugation Kit

     

    Product Videos

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