CellMosaic’s maleimide activated Streptavidin ML is designed for direct conjugation of any biopolymer or small molecule containing free sulfhydryl groups for use in biotin-streptavidin based assay. Streptavidin has been modified with BMPS (N-(β-maleimidopropyloxy) succinimide ester) to attach 2-4 maleimide groups per streptavidin molecule.  

Application

• Suitable for loading thiol biopolymers or small molecules onto streptavidin (solution or immobilized) for assay
• Easy to adjust the loading of biopolymers or small molecules based on the amount added for optimal signal

Key Features of this Maleimide Activated Streptavidin

• Lyophilized powder and ready for conjugation reaction after reconstitution with water, no need for external buffer
• Medium loading with 2-4 average maleimide groups per streptavidin

Example Data

Example 1: MS analysis of Streptavidin (left) and Maleimide activated Streptavidin ML (Lot#:0291031. Number of Maleimide groups: 2.9) (right) (data acquired by MDS SCIEX 4800 MALDI TOF Analyzer). 

Example 2: MS analysis of Streptavidin (left) and Maleimide activated Streptavidin ML (Lot#:0291032. Number of Maleimide groups: 2.9) (right) (data acquired by MDS SCIEX 4800 MALDI TOF Analyzer).