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C6 Maleimide Activated HRP

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Price:
$128.00
SKU:
CM53214-1x2mg
Availability:
In stock, usually ships within 24 hrs
Shipping:
Calculated at checkout
Quantity:


This is a replacement product for CM53211.  

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Product Sheet

COA-022521

CellMosaic’s C6 maleimide activated horseradish peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing free sulfhydryl groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with EMCS (N-Ɛ-maleimidocarproyl oxysuccinimide ester, 9.4 Å spacer length) to attach 1 to 2 maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to sulfhydryl-containing compounds to generate the majority single labeled HRP-conjugates reporter probes. 

C6 Maleimide activated HRP is much more stable than previously offered product CM53211.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Application

  • Couple HRP to antibodies and other proteins for western blotting or ELISA
  • Couple HRP to oligonucleotides for in-situ hybridization

Key Features of this Maleimide activated HRP

  • Lyophilized powder ready for conjugation after reconstitution with water, no need for external buffer.
  • Suitable for single labeling with an average maleimide groups per HRP of 1 to 2
  • High purity: ≥ 99% by SEC HPLC 
  • High activity: >350 units/mg protein

References

  • Hashida, S., et al. (1984). More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. J. Appl. Biochem. 6, 56-63.
  • Imagawa, M., et al.(1982). Characteristics and evaluation of antibody-horseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and periodate. J. Appl. Biochem. 4, 41-57.
  • O’Sullivan, M.J., et al. (1979). Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay. Anal. Biochem. 100, 100-108.

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