C6 Maleimide Activated HRP

Product Description

This is a replacement product for CM53211.  

CellMosaic’s C6 maleimide activated horseradish peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing free sulfhydryl groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with EMCS (N-Ɛ-maleimidocarproyl oxysuccinimide ester, 9.4 Å spacer length) to attach 1 to 2 maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to sulfhydryl-containing compounds to generate the majority single labeled HRP-conjugates reporter probes. 

C6 Maleimide activated HRP is much more stable than previously offered product CM53211.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Application

• Couple HRP to antibodies and other proteins for western blotting or ELISA
• Couple HRP to oligonucleotides for in-situ hybridization

Key Features of this Maleimide activated HRP

• Lyophilized powder ready for conjugation after reconstitution with water, no need for external buffer.
• Suitable for single labeling with an average maleimide groups per HRP of 1 to 2
• High purity: ≥ 99% by SEC HPLC 
• High activity: >350 units/mg protein

References

• Hashida, S., et al. (1984). More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. J. Appl. Biochem. 6, 56-63.
• Imagawa, M., et al.(1982). Characteristics and evaluation of antibody-horseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and periodate. J. Appl. Biochem. 4, 41-57.
• O’Sullivan, M.J., et al. (1979). Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay. Anal. Biochem. 100, 100-108.