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Peptide/Protein—KLH Conjugate

  • Image 1
  • Scheme: Thiol peptide reaction with a maleimide activated carrier protein
  • Example 1: Size exclusion HPLC analysis of protein-KLH conjugates
Price:
Please contact us for a quote.
SKU:
BC0009


Product Description

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies. Low molecular weight haptens such as peptides, small proteins and drug molecules are conjugated to the KLH routinely at CellMosaic.

  • Chemistry:  Peptides/proteins are loaded onto KLH (keyhole limpet hemocyanin) through its surface amines.
  • Specification: A pool of heterogeneous products.
  • Procedure:  After a buffer exchange (if necessary), labeling of KLH using NHS ester-maleimide or other suitable heterobifunctional crosslinkers followed by gel filtration to remove excess crosslinking reagents.Then conjugation of peptide/protein to labeled KLH and gel filtration. Finally concentration determination and gel electrophoresis analysis of the peptide/protein-KLH conjugates.
  • Price: please contact us for a quote.
  • Starting materials: Please check biopolymer for ordering information.
  • Discount: 15 % discount price applies to additional conjugates ordered at the same time.

Examples (see images):

  1. Synthesis of protein-KLH conjugates at CellMosaic: loaded 25 proteins per KLH.  bc0009-protein-klh.jpg

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