Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single purification step typically provides the resulting KLH-peptide at greater than 95% purity.

This kit provides materials to perform KLH labeling of one to three peptides. Total amount of activated KLH included in a reaction: 2 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.

Requirement for Cys peptide:

• Amount: 0.10–0.51 µmol (20–100 equivalents of peptide over KLH)
• HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
• The Cys peptide should be stored at -80 degrees Celsius.
• HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this KLH-Peptide Conjugation Kit:

• High quality maleimide-activated KLH for the conjugation: >95% purity 
• High maleimide groups per KLH for peptide loading: 50-100
• A single purification step affords over 95% of the KLH-peptide conjugates 
• Fast preparation: less than 1 h hands-on time
• All reagents included, from preparation to purification
• Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.