Loading... Please wait...

Our Newsletter


KLH-Peptide Conjugation Kit

  • Peptide KLH conjugates
  • Conjugation scheme
  • Schematic diagram of the workflow
Price:
$340.00
SKU:
CM52411x1
Brand:
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Peptide Information: MW, sequence:

(Maximum Size: . Type: )
Quantity:


 

Download Documents
SDS USER MANUAL
COA_Activated KLH Lots11.0126

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single purification step typically provides the resulting KLH-peptide at greater than 95% purity.

This kit provides materials to perform KLH labeling of one to three peptides. Total amount of activated KLH included in a reaction: 2 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Requirement for Cys peptide:

  • Amount: 0.10–0.51 µmol (20–100 equivalents of peptide over KLH)
  • HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
  • The Cys peptide should be stored at -80 degrees Celsius.
  • HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this KLH-Peptide Conjugation Kit:

  • High quality maleimide-activated KLH for the conjugation: >95% purity 
  • High maleimide groups per KLH for peptide loading: 50-100
  • A single purification step affords over 95% of the KLH-peptide conjugates 
  • Fast preparation: less than 1 h hands-on time
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.

Add to Wish List

Click the button below to add the KLH-Peptide Conjugation Kit to your wish list.

You Recently Viewed...