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KLH-Peptide Conjugation Kit

  • Peptide KLH conjugates
  • Conjugation scheme
  •  Schematic diagram of the workflow
Price:
$286.00
SKU:
CM52411x1
Brand:
Availability:
In stock, usually ships within 1-3 days
Shipping:
Calculated at checkout
Peptide Information: MW, sequence:

(Maximum Size: . Type: )
Quantity:


 

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SDS USER MANUAL
COA_Activated KLH Lots11.0126

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single purification step typically provides the resulting KLH-peptide at greater than 95% purity.

This kit provides materials to perform KLH labeling of one to three peptides. Total amount of activated KLH included in a reaction: 2 mg.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


Requirement for Cys peptide:

  • Amount: 0.10–0.51 µmol (20–100 equivalents of peptide over KLH)
  • HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
  • The Cys peptide should be stored at -80 degrees Celsius.
  • HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this KLH-Peptide Conjugation Kit:

  • High quality maleimide-activated KLH for the conjugation: >95% purity 
  • High maleimide groups per KLH for peptide loading: 50-100
  • A single purification step affords over 95% of the KLH-peptide conjugates 
  • Fast preparation: less than 1 h hands-on time
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.

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