Digoxigenin is a steroid isolated from Digitalis plants and frequently used for biodetection purpose. This KLH-digoxigenin conjugate is designed with a flexible linker to allow easy interaction between digoxigenin and other detection molecules, and is synthesized at CellMosaic for our customers' usage in immunization or immunoassay. A linker is first added to digoxigenin site-specifically via its OH group, and then digoxigenin is labeled at the surface amines of KLH. The final conjugate is lyophilized from phosphate buffered saline containing sugar-based stabilizer for easy shipping and storage. 

The product is sold as either 1 vial of 1 mg (Cat# CM52108_1MG) or 5 vials of 1 mg (Cat# CM52108_5MG). For bulk orders, please contact us for a quote.

Chemical Information

• Chemical name: KLH-Digoxigenin Conjugate
• Chemical formula: N/A
• Molecular weight: 400KDa;           CAS number: N/A

Specification

• Physical appearance: white to off-white preservative-free lyophilized powder in a 2.0 mL centrifuge tube
• Storage temp.: -20 °C
• Purity: ≥ 99 % of conjugates by HPLC
• Digoxigenin over KLH ratio: 30~40

References

Selected few references using digoxigenin detection system:

1. Martin R. et. al. A highly sensitive, nonradioactive DNA labeling and detection system. Biotechniques. 1990, 9, 762-768.
2. Bautista J.; Mateos-Nevado MD. Immunological detection and quantification of oxidized proteins by labelling with digoxigenin. Biosci Biotechnol Biochem 1998, 62, 419-423.
3. Heyduk E.; Hickey R.; Pozzzi N.; Heyduk T. Peptide ligand-based ELISA reagents for antibody detection. Anal Biochem. 2018, 559, 55-61.
4. Komminoth P. Digoxigenin as an alternative probe labeling for in situ hybridization. Diagn Mol Pathol. 1992, 1, 142-150.