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KLH-Peptide Conjugation Kit

  • Peptide KLH conjugates
  • Peptide KLH conjugation scheme
Price:
$210.00
SKU:
CM52411x1
Availability:
Custom kit made to order, usually ships within 1 week
Shipping:
Calculated at checkout
Peptide Information: MW, sequence:

(Maximum Size: . Type: )
Quantity:


 

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SDS USER MANUAL

Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. KLH is a large, oxygen-carrying, multi-subunit protein that contains chelated copper of non-heme origin. CellMosaic® has designed this personalized KLH conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepare the KLH-peptide conjugate by reacting their peptide (supplied by the customer) with activated KLH. A single purification step typically provides the resulting KLH-peptide at greater than 95% purity.

This kit provides materials to perform KLH labeling of one to three peptides. Total amount of activated KLH included in a reaction: 2 mg.


Requirement for Cys peptide:

  • Amount: 1-5 mg depending on the peptide MW
  • HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present. The Cys peptide should be stored at -80 degrees Celsius.
  • HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this KLH-Peptide Conjugation Kit:

  • High quality maleimide-activated KLH for the conjugation: >95% purity 
  • Optimal maleimide groups per KLH for peptide loading: 10-20
  • A single purification step to remove free peptides 
  • Fast preparation: less than 1 h hands-on time
  • All reagents included, from preparation to purification
  • Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features.

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.

 

 

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