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SDS | USER MANUAL |
CellMosaic® has designed this personalized antibody peptide conjugation kit to label any IgG antibody with any peptide containing a reactive Cys group using BMPS crosslinker. The user supplies the antibody and peptide. The kit includes BMPS, which can be coupled first to the antibody to introduce the maleimide groups on the surface, and then the maleimide groups react with the Cys of the peptide to form the conjugates. The product is purified to remove any unreacted drugs.
This kit provides materials to perform one to three conjugations. Scale of the reaction based on antibody: 3 mg
Requirement for Cys peptide:
Potential interfering compounds for labeling and conjugation reactions:
Thiols: e.g., DTT and mercaptoethanol
Requirement for antibody (IgG):
Preferably >90% pure by gel electrophoresis
Key Features of this Antibody Peptide Conjugation Kit:
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.