CellMosaic® has designed this personalized antibody peptide conjugation kit to label any IgG antibody with any peptide containing a reactive Cys group using BMPS crosslinker. The user supplies the antibody and peptide. The kit includes BMPS, which can be coupled first to the antibody to introduce the maleimide groups on the surface, and then the maleimide groups react with the Cys of the peptide to form the conjugates. The product is purified to remove any unreacted drugs.

This kit provides materials to perform one to three conjugations. Scale of the reaction based on antibody: 3 mg

Requirement for Cys peptide:

• Amount: 0.6 µmol
• HPLC purified and lyophilized. Please make sure there is no reducing reagents, such as DTT are present. The Cys peptide should be stored at -80 degrees Celsius.
• HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide 

Potential interfering compounds for labeling and conjugation reactions:

Thiols: e.g., DTT and mercaptoethanol

Requirement for antibody (IgG):

• Amount: 3 mg (protein content) 

• Preferably >90% pure by gel electrophoresis

Key Features of this Antibody Peptide Conjugation Kit:

• Offers a simple and easy way to label IgG with peptide
• Fast and easy preparation: 4 h preparation and <2 h hands-on time
• Target 8-10 peptides per antibody
• All reagents and supplies included for preparation to purification
• >95% conjugated products (free of or <5% unreacted peptide)
• Options to choose tailored services at CellMosaic prior to and after conjugation

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates.