Ova-Peptide Conjugation Kit

Product Description

Ovalbumin (Ova) is a small protein sometimes used as a carrier protein for antibody production. Chicken egg albumin is the major protein constituent of egg whites and has a MW of 44.3 KDa if the carbohydrate and phosphate portions are accounted. CellMosaic® has designed this personalized Ova conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepares the Ova-peptide conjugate by reacting their peptide (supplied by the customer) with activated Ova. A single purification step typically provides the resulting Ova-peptide at greater than 95% purity.

This kit provides materials to perform Ova labeling of one (CM52418x1) or three peptides (CM52418x3). Total amount of activated Ova included in a reaction: 2 mg.

Requirement for Cys peptide:

• Amount: 0.23–0.9 µmol (5–20 equivalents of peptide over Ova).
• HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
• The Cys peptide should be stored at -80 degrees Celsius.
• HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

Key Features of this Ova-Peptide Conjugation Kit:

• High quality maleimide-activated Ova for the conjugation: >95% purity 
• High maleimide groups per Ova for peptide loading: 10-20
• A single purification affords over 95% of the Ova-peptide conjugates 
• Fast preparation: less than 1 h hands-on time
• All reagents included, from preparation to purification
• Options to choose tailored services at CellMosaic prior to and after conjugation

Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);

After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.