Ovalbumin (Ova) is a small protein sometimes used as a carrier protein for antibody production. Chicken egg albumin is the major protein constituent of egg whites and has a MW of 44.3 KDa if the carbohydrate and phosphate portions are accounted. CellMosaic® has designed this personalized Ova conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, customers prepare the Ova-peptide conjugate by reacting their peptide (supplied by the customer) with activated Ova (supplied in the kit). A single purification step typically provides the resulting Ova-peptide at greater than 95% purity.
This kit provides materials to perform Ova labeling of one (CM52418x1) or three peptides (CM52418x3). Total amount of activated Ova included in a reaction: 2 mg.
Requirement for Cys peptide:
- Amount: 0.23–0.9 µmol (5–20 equivalents of peptide over Ova).
- HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
- The Cys peptide should be stored at -80°C .
- HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
Key Features of this Ova-Peptide Conjugation Kit:
High quality maleimide-activated Ova for the conjugation: >95% purity
High maleimide groups per Ova for peptide loading: 10-20
A single purification affords over 95% of the Ova-peptide conjugates
Fast preparation: less than 1 hour hands-on time
All reagents included, from preparation to purification
- Options to choose tailored services at CellMosaic® prior to and after conjugation
Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);
After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.