Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. BSA is often used as a carrier protein for immunization. CellMosaic® has designed this personalized BSA conjugation kit to work with any peptide containing a reactive Cys group. The user supplies its own peptide. Using the kit components, the customer prepares the BSA-peptide conjugate by reacting their peptide (customer supplied) with activated BSA. One step purification typically provides the resulting BSA-peptide at greater than 95% purity.
This kit provides materials to perform BSA labeling of one to three peptides. Total amount of activated BSA included in a reaction: 2 mg.
Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.
Requirement for Cys peptide:
- Amount: 0.15–0.6 µmol (5–20 equivalents of peptide over BSA)
- HPLC purified and lyophilized. Please make sure there is no reducing reagents, such as DTT are present.
- The Cys peptide should be stored at -80 degrees Celsius.
- HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
Key Features of this BSA-Peptide Conjugation Kit:
High quality maleimide activated BSA for the conjugation: >95% purity
Optimal maleimide groups per BSA for peptide loading: 20–40 (please check the COA of each individual batch for the exact loading)
A single purification affords over 95% of the BSA-peptide conjugates
Fast preparation: less than 1 h hands-on time
All reagents included, from preparation to purification
- Options to choose tailored services at CellMosaic prior to and after conjugation
Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic will give recommendations for the conjugation if your peptide has special features (complementary service);
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.