Product Description
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COA of Activated BSA Lot S338.S8.052421 |
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Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. BSA is often used as a carrier protein for immunization. CellMosaic® has designed this personalized BSA conjugation kit to work with any peptide containing a reactive Cys group. The user supplies their own peptide. Then, using the kit components, prepares the BSA-peptide conjugate by reacting their peptide (customer supplied) with activated BSA. A one-step purification process typically provides the resulting BSA-peptide conjugate at greater than 95% purity.
This kit provides materials to perform BSA labeling of one (CM52402x1) or three (CM52402x3) peptides. Total amount of activated BSA included in a reaction: 2 mg.
Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.)
Requirement for Cys peptide:
- Amount: 0.15–0.6 µmol (5–20 equivalents of peptide over BSA)
- HPLC purified and lyophilized. Please make sure there are no reducing reagents, such as DTT are present.
- The Cys peptide should be stored at -80°C.
- HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
Key Features of this BSA-Peptide Conjugation Kit:
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High quality maleimide activated BSA for the conjugation: >95% purity
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Optimal maleimide groups per BSA for peptide loading: 20–40 (please check the COA of each individual batch for the exact loading)
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A single-step purification affords over 95% BSA-peptide conjugates
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Fast preparation: less than 1 hr of hands-on time
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All reagents included from preparation to purification
- Options to choose tailored analytical services at CellMosaic® prior to and after conjugation
Prior to conjugation: you can supply your peptide information when you place your order. CellMosaic® will give recommendations for the conjugation if your peptide has special features (complementary service);
After conjugation: you can choose to send your sample to CellMosaic® for HPLC analysis of the conjugates or peptide samples for determination of the peptide loading.
