CellMosaic® has designed this personalized conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepares the HRP-peptide conjugate by reacting their peptide (customer supplied) with activated HRP. One-step purification typically removes most of the unreacted peptides.
HRP contains two available primary Ɛ-amine groups for modification. The maleimide activated HRP via surface amine has limited maleimides (average 1-2 maleimides per HRP) and is suitable for single labeling. Depending on the activity of your peptide, some unreacted HRP may be present in the final mixture. For typical ELISA and other solid based immunoassays, the unreacted HRP can be washed away after peptide binding. For other applications that require complete removal of free HRP, column purification may be used to remove any unreacted HRP.
This kit provides materials to label one (CM32401x1) to three (CM32401x3) peptides. Total amount of activated HRP included in one reaction: 2 mg.
Requirement for Cys peptide:
- Amout: 1-5 mg depending on the peptide MW
- HPLC purified and lyophilized. Please make sure there is no reducing reagents, such as DTT, are present. The Cys peptide should be stored at -80 degress Celsius.
- HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
Key Features of this HRP-Peptide Conjugation Kit:
High quality maleimide-activated HRP for the conjugation: >99% purity and >350 units/mg protein activity
Optimal maleimide groups per HRP for single-label peptide: 1.5 for typical batch
A single purification removes the majority of unreacted peptides
Fast preparation: less than 1 h hands-on time
Options to choose tailored services at CellMosaic prior to and after conjugation
Prior to conjugation: supply your peptide information when you place your order and CellMosaic will give recommendations for the conjugation based on your peptide's special features
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates