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SDS | USER MANUAL |
CellMosaic® has designed this personalized conjugation kit to work with any peptide containing a reactive Cys group. Using the kit components, the customer prepares the HRP-peptide conjugate by reacting their peptide (customer supplied) with activated HRP. One-step purification typically removes most of the unreacted peptides.
HRP contains two available primary Ɛ-amine groups for modification. The maleimide activated HRP via surface amine has limited maleimides (average 1-2 maleimides per HRP) and is suitable for single labeling. Depending on the activity of your peptide, some unreacted HRP may be present in the final mixture. For typical ELISA and other solid based immunoassays, the unreacted HRP can be washed away after peptide binding. For other applications that require complete removal of free HRP, column purification may be used to remove any unreacted HRP.
This kit provides materials to label one to three peptides. Total amount of activated HRP included in a reaction: 2 mg.
Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.
Requirement for Cys peptide:
Key Features of this HRP-Peptide Conjugation Kit:
Prior to conjugation: you can supply your peptide information when you place your order, and CellMosaic will give recommendations for the conjugation if your peptide has special features
After conjugation: you can choose to send your sample to CellMosaic for HPLC analysis of the conjugates
Labeling Chemistry