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Maleimide Activated HRP

  • Maleimide activated Horseradish Peroxidase
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Product Sheet



CellMosaic’s maleimide activated Horseradish Peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing free sulfhydryl groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with BMPS (N-(β-Maleimidopropyloxy) succinimide ester) to attach 1-2 maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to sulfhydryl-containing compounds to generate the majority single labeled HRP-conjugates reporter probes. 

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  • Couple HRP to antibodies & other proteins for Western Blotting & ELISA
  • Couple HRP to oligonucleotides for in-situ hybridization

Key Features of this Maleimide activated HRP

  • Lyophilized powder and ready for conjugation reaction after reconstitution with water, no need for external buffer.
  • Suitable for single label, average maleimide groups per HRP: 1-2
  • High purity: ≥ 99% by SEC HPLC 
  • High activity: >200 units/mg protein


  • Hashida, S., et al. (1984). More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. J. Appl. Biochem. 6, 56-63.
  • Imagawa, M., et al.(1982). Characteristics and evaluation of antibody-horseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and periodate. J. Appl. Biochem. 4, 41-57.
  • O’Sullivan, M.J., et al. (1979). Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay. Anal. Biochem. 100, 100-108.

Example Data

Example 1: MALDI-TOF MS analysis of HRP (left) and Maleimide activated HRP (Lot#:s7.030918. Number of Maleimide groups: ~2) (right) (Intact MS). 

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