Product Description
| Download Documents | |
| SDS | Product Sheet |
| COA_Lot S356.S9.090421 | |
CellMosaic’s AqT® biotin antibody product is prepared by labeling the surface amines of the antibody with a super-hydrophilic AqT™ linker with exceptional high loading. AqT® linkers are novel proprietary biomaterials invented at CellMosaic® that are chemically assembled from a class of natural and edible sugar alcohol compounds with properties by design.
AqT® linker used to prepare this product is flexible with >20 atoms length. This long flexible hydrophilic spacer prevents the crowding and interaction of streptavidin with the antibody, increasing the sensitivity of detection.
Description of the Materials
- Product Name: AqT® Biotin G/H IgG, Fc gamma fragment specific
- Labeling Chemistry: Biotin is labeled at the surface amines of the antibody via a long and flexible super-hydrophilic AqT® linker (>20 atoms length)
- Molecule Weight: 154KDa
- Physical Form: White to off-white lyophilized powder in a centrifuge tube.
- Amount: each tube contains 100 μg of AqT® Biotin G/H IgG
Antibody Information
- Antibody Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non- immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
- Host/Isotype: Goat
- Class: Polyclonal
- Type: Secondary Antibody
Purity Specifications and Results
- Purity: Free of any unreacted AqT® biotin
- Purification Method: The antibody was purified by immunoaffinity chromatography. After biotinylation, the product was desalted to remove any unreacted AqT® biotin.
- Biotin Loading: average 4 to 6 per antibody (see COA for specific lot)
Usage
- Reconstitution: Dissolve in 100 μL of deionized water to obtain 1 mg/mL solution in 1xPBS buffer containing 2 mg/mL of BSA.
- Preservative: None
- Suggested Working Concentration or dilution Range (actual dilution used must be determined experimentally)
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Applications |
Dilution (Suggested) |
|
ELISA and Western Blot (WB) |
1:20,000-1,400,000 |
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Enzyme immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP) |
1:500-1:5,000 |
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Flow Cytometry |
1:200-1:1,000 |
Frequently Asked Questions:
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Other Details
The information in this document is subject to change without notice. CellMosaic® assumes no responsibility for any errors or omissions that may appear in this document. Under no circumstances shall CellMosaic be liable, whether in contract, tort, warranty, or under any statute or on other legal theory, for special, incidental, indirect, punitive, multiple, or consequential damages related to or arising from this document, including but not limited to the use of this product thereof.CellMosaic’s products, including but not limited to AqT®, NeIon™, oxLink™ and sxLink™ (collectively, the “Products”), are covered by, or are the subject of, one or more issued patents and pending patent applications, including without limitation: 9,688,663 B2; 8,907,079 B2; 9,511,150 B2; 9,907,854 B2; 9,346,833 B2; 9,518,067 B2; CN104066451B; CN107043339B; CA 2,841,313; EP 2734238 B1; AU2012284055; JP 6240599 B2; and corresponding foreign patents and patent applications. The purchase of the Product conveys solely a limited, non-exclusive, non-transferable, non-sublicensable license to use the Product only for the purchaser’s internal research and development purposes. No right or license is granted, either expressly or by implication, estoppel, or otherwise, under any patent, trademark, copyright, or other intellectual property right of CellMosaic, except as expressly stated herein. Any commercial use of the Product is strictly prohibited without a separate written agreement from CellMosaic. Prohibited commercial uses include, without limitation: (a) the sale, lease, licensing, distribution, or other transfer of the Product or any materials derived from or produced using the Product; (b) the sale, lease, licensing, or other grant of rights to use the Product or any materials derived from or produced using the Product; and (c) the use of the Product to perform services for a fee or other consideration for third parties, including without limitation contract research, screening services, or diagnostic or therapeutic applications. For information regarding commercial licensing or collaboration, please contact us at info@cellmosaic.com.
