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Maleimide Activated HRP

  •  Maleimide activated Horseradish Peroxidase
Price:
$101.00
SKU:
CM53211
Availability:
On stock, usually ships within 24 hrs
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Product Description

 

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Product Sheet COA Example

CellMosaic’s maleimide activated Horseradish Peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing free sulfhydryl groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with BMPS (N-(β-Maleimidopropyloxy) succinimide ester) to attach 1-3 maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to sulfhydryl-containing compounds to generate the majority single labeled HRP-conjugates reporter probes. 


Application

  • Couple HRP to antibodies & other proteins for Western Blotting & ELISA
  • Couple HRP to oligonucleotides for in-situ hybridization

Key Features of this Maleimide activated HRP

  • Lyophilized powder and ready for conjugation reaction after reconstitution with water, no need for external buffer.
  • Suitable for single label, average maleimide groups per HRP: 1-3
  • High purity: ≥ 97%
  • Activity: >240 units/mg protein

References

  • Hashida, S., et al. (1984). More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. J. Appl. Biochem. 6, 56-63.
  • Imagawa, M., et al.(1982). Characteristics and evaluation of antibody-horseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and periodate. J. Appl. Biochem. 4, 41-57.
  • O’Sullivan, M.J., et al. (1979). Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay. Anal. Biochem. 100, 100-108.

 

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