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    Main Menu

  • About Us
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      • Terms and Conditions for Sales of Products
    • Ordering Services
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  • Technologies
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    • Advanced Conjugation Processes
    • NeIon™ Technologies
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    • Custom Conjugation Guide
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  • Antibody-Drug Conjugate (ADC) Kits and Solution
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    • ADC Controls
    • Buffers and others
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    • Biotin and Streptavidin Labeling
    • Others
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    • Mass Tags
    • Modified Biomolecules and Conjugates
    • Others
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    • Drug Conjugate Synthesis
  • Services
    • Custom Bioconjugation Service
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    • Bioconjugate Drug Development
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    • Biologics, Bioconjugate Analysis and Purification
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  • Thiol activated HRP (HRP-PEG4-SPDP)
  • Thiol activated HRP (HRP-PEG4-SPDP)

    Thiol Activated HRP (HRP-PEG4-SPDP)

    MSRP:
    Now: $177.00
    (You save )
    SKU:
    CM53101-1x2mg
    Availability:
    In stock, usually ships within 24 hrs
    Shipping:
    Calculated at Checkout

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    • Overview

    Product Description

     

    Download Documents
    Product Sheet

    COA-s71208

    CellMosaic’s thiol activated Horseradish Peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing maleimide, haloacetyl, and thiol groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with 1-3 pyridyl disulfide groups per HRP molecule with a long PEG spacer. The pyridyl disulfide of the HRP needs to be reduced to produce the free thiol groups prior reacting with maleimide and haloacetyl derivatives. But it can be also used to react directly with -SH groups of the proteins, peptides, or other ligands to form reversible disulfide linkages. Upon conjugation, the activated HRP will be covalently attached to the sulfhydryl-containing compounds to generate primarily single labeled HRP-conjugate reporter probes.

    Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.) or 2-day delivery (ships Mon.-Wed.) at checkout.


    Application

    • Couple HRP to antibodies & other proteins for Western Blotting & ELISA
    • Couple HRP to oligonucleotides for in-situ hybridization

    Key Features of this Maleimide activated HRP

    • Lyophilized powder and ready to be reduced by DTT or TCEP after reconstitution with water, no buffer is needed.
    • Suitable for single label, average disulfide groups per HRP: 1-3
    • High purity: ≥ 99%
    • Activity: >200 units/mg protein

     

     

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