Loading... Please wait...

Products and Services

Our Newsletter


Surface Activation

This is an intermediate step that prepares functionalized solid support for biopolymer immobilization. Some of the solid supports are available commercially in active form . For non-active solid supports, we can either convert them to an activate form first or let them react directly to a biopolymer using active coupling reagents.

CellMosaic uses these processes exclusively for supporting our custom Biopolymer Immobilization.

Example 1: CNBr activation of agarose.

  • Description
  • Scheme
  • References

 

The preparation of active esters of hydroxyl-containing solid supports for reaction with amine-containing biopolymers or small molecules.

  • Chemistry: Cyanogen bromide reacts with hydroxyl groups to form a cyanate ester.
  • Starting materials: Solid supports containing hydroxyl groups such as agarose.
  • Note: CNBr-activate solid supports produced in CellMosaic will be used directly for the large scale immobilization of antigens/antibodies due to the stability issue of active cyanate esters.

 

Activation of solid supports by BrCN

 

 

CNBr activation of solid support

 

 

1. Axén, R.; Porath, J.; Ernback, S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967, Jun 24;214(5095):1302-4.

2. Porath, J.; Aspberg, K.; Drevin, H.; Axén, R. Preparation of cyanogen bromide-activated agarose gels. J Chromatogr. 1973, 86, 53-65.

3. March, S. C.; Parikh, I; Guatrecasas, P. A. Simplified method for cyanogen bromide activation of agarose for affinity chromatography. Analytical Biochem. 1974, 60, 149-152.

4. Kohn, J.; Wilchek, M. A colorimetric method for monitoring activation of sepharose by cyanogen bromide. Biochem Biophys Research Communications 1978, 84, 7-14.