Product Description
| Download Documents | |
| Product Sheet |
|
| SDS | COA_Lot# NB0320722 |
S-methyl DM4 is the primary cellular or liver metabolite of ADC or other conjugates prepared with DM4. DM4 (ravtansine) is a semisynthetic analogue of maytansine with a C3 ester side chain bearing a dimethyl hindered thiol substituent. It binds to tubulin and inhibits the assembly of microtubules. DM4 is used to prepare antibody-drug conjugates. DM4 is not very stable in aqueous solution and can be oxidized or dimerized. CellMosaic’s S-methyl DM4 is a high purity product that can serve as a standard for HPLC and LC-MS/MS analysis. The product is formulated in DMSO solution, quantified by UV/HPLC using DM1 as standard/calibrator at 252 nm, and ready to use after dilution. Although S-methyl DM4 inhibits polymerization weaker than DM4, it can also be used as a stable control for the ADC studies instead of DM4.
The product is sold as 1 vial of 1 mg (126 uL x 10 mM). If you would like to buy multiple vials, please check our Bulk Pricing:Buy in bulk and save
Chemical Information
- Chemical name: S-Methyl DM4 (Ravatansine)
- Chemical formula: C39H56ClN3O10S
- Molecular weight: 794.40 Da
- CAS number: 890654-03-2
Specification
- Physical appearance: solution
- Storage temp.: -20 °C
- Purity: ≥ 99 % by C18 HPLC
- Concentration: 10 mM in DMSO (determined by UV/HPLC using DM1 as standard/calibrator at 252 nm)
References
- Erickson, H. K. al. (2010) Tumor Delivery and In Vivo Processing of Disulï¬de-Linked and Thioether-Linked Antibody-Maytansinoid Conjugates. Bioconjugate Chem. 21, 84-92.
- Widdison, W. et al. (2015) Metabolites of Antibody−Maytansinoid Conjugates: Characteristics and in Vitro Potencies. Pharmacuetics, 12, 1762-1773.
- Lopus M. et al. (2010). Maytansine and Cellular Metabolites of Antibody-Maytansinoid Conjugates Strongly Suppress Microtubule Dynamics by Binding to Microtubules. Mol. Cancer. Ther. 9(1), 2689-2699.
- Y. et al. (2022). Sensitive LC-MS/MS quantification of unconjugated maytansinoid DM4 and its metabolite S-methyl-DM4 in human plasma. Bioanalysis. 14(6), 357-368.
